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|Name:||TOOS||CAS No.:||82692- 93-1|
|Appearance:||White Crystal Powder||Purity:||>99%|
|Molecular Weight:||295.33||Molecular Formula:||C12H18NNaO4S|
82692- 93-1 Trinder Reagent,
TOOS Trinder Reagent,
CAS NO 82692- 93-1
Trinder's Reagent;TOOS;CAS82692- 93-1|;Purity ≥ 99%;N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline
What are the advantages of TOOS color reagent developed and produced by Desheng
TOOS color reagent is a new type of color reagent, which is widely used in diagnostic testing and biochemical tests. The TOOS chromogenic reagent developed by Desheng has several advantages over conventional chromogenic reagents in the colorimetric determination of hydrogen peroxide activity. It not only has higher molar absorbance, but also has higher color reaction sensitivity, and can carry out some trace amounts of serum. The measurement of the item to be tested, and the color reaction product is stable and not easy to fade. Because the color reaction of the color reagent was first proposed by Trinder in 1969, it was named Trinder reaction. In fact, it was not originally used as a new color reagent as a color reagent, but phenol was used. Later, many people proposed that it could be more sensitive. The compound replaces phenol, which significantly improves the sensitivity of the reaction and the detection results are more accurate.
|substrate||Absorption wavelength λ max(nm)||PH range of solution||Molar absorbance||purity|
TOOS color reaction
The color reaction principle of TOOS color reagent: TOOS color reagent is a highly water-soluble aniline derivative. In the presence of hydrogen peroxide and peroxidase, the new Trinder's reagent is used in the oxidative coupling reaction process with 4-aminoantipyrine (4-AA) or 3-methylbenzothiazole sulfone hydrazone (MBTH) in Chemicalbook , Forming a very stable purple or blue dye. The molar absorbance of the dye coupled with MBTH is 1.5-2 times higher than that of the dye coupled with 4-AA; however, the 4-AA solution is more stable than the MBTH solution. The substrate is oxidized enzymatically by its oxidase to produce hydrogen peroxide. The concentration of hydrogen peroxide corresponds to the concentration of the substrate. Therefore, the amount of the substrate can be determined by the color development of the oxidative coupling reaction. Glucose, alcohol, acyl-CoA and cholesterol can be used to detect those substrates coupled with the new Trinder's reagent and 4-AA. There are 10 new Trinder's reagents. Among the new Trinder's reagents, TOOS is the most commonly used. However, for specific substrates, testing different kinds of new Trinder's reagents is necessary to develop the best detection system.
When purchasing color reagents, pay special attention to the product's purity, sensitivity, appearance and other parameters. The TOOS color reagents produced by Desheng not only have a purity of >99%, high sensitivity, and ensure that the appearance is white crystalline powder, but also the absorption wavelength, color reaction intensity and It has good performance in terms of fading, so TOOS is the most commonly used color reagent, and it is also the color reagent that Desheng currently cooperates with. It is precisely because the company's products are of good quality, strong stability, high sensitivity, and preferential prices. , So it has been widely praised by domestic and foreign customers, and the repurchase rate is as high as 98%.
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