Chromogenic substrate TOOS 82692-93-1: The 'invisible hero' in IVD detection

In the field of in vitro diagnostics (IVD), chromogenic substrates serve as key reagents that directly affect the sensitivity and accuracy of detection results. Among them, TOOS reagent (N-ethyl-N - (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt) has become the "hidden hero" of blood glucose, uric acid, liver function and other detection items due to its high water solubility, sensitivity and stability.

TOOS: The 'star member' of the new Trinder's reagent

TOOS is a novel Trinder's reagent that has higher water solubility compared to traditional phenol and aniline based chromogenic substrates. It can reduce the risk of precipitation or crystallization and has significant absorbance at a wavelength of 590nm, ensuring the accuracy of detection results. The color reaction is based on the Trinder reaction mechanism: in the presence of hydrogen peroxide (H ₂ O ₂), TOOS couples with 4-aminoantipyrine (4-AAP) and peroxidase (POD) to form a red quinone imine compound, and the color intensity is positively correlated with the concentration of the target substance. This characteristic makes it widely applicable in IVD detection.

TOOS powder

Core application scenarios of TOOS in IVD detection

1. Blood glucose detection: "accurate scale" of diabetes management

TOOS is the core component of glucose detection kits. Under the catalysis of glucose oxidase, glucose is oxidized to form H ₂ O ₂, which then undergoes a color reaction with TOOS to produce a purple product. By detecting the absorbance at a wavelength of 590nm, the blood glucose concentration can be quantitatively analyzed.

2. Uric acid testing: a "sensitive indicator" for renal function assessment

Uric acid is an end product of purine metabolism, and its concentration changes reflect the renal function status. Under the catalysis of uricase, uric acid is hydrolyzed into allantoin and H ₂ O ₂. TOOS generates red quinone imine compounds through the Trinder reaction, achieving quantitative detection of uric acid concentration.

3. Liver function testing: the "sensitive sentinel" for diagnosing liver injury

TOOS plays a crucial role in adenosine deaminase (ADA) detection. ADA catalyzes the deamination of adenine nucleotides to produce hypoxanthine, which is then catalyzed by hypoxanthine oxidase (XOD) to produce uric acid and H ₂ O ₂, triggering the TOOS colorimetric reaction.

4. Blood lipid testing: a "warning signal" for metabolic syndrome

TOOS is also used in the detection of triglycerides and cholesterol. By enzymatic reaction, H ₂ O ₂ is generated, and the TOOS color product shows a linear relationship with the concentration of the target substance, which can achieve accurate quantification of blood lipid levels.

Technical advantages and clinical value of TOOS

1. High water solubility: simplifies the operation process

The high water solubility of TOOS allows it to dissolve directly in water without the need for organic solvents, significantly reducing the difficulty of reagent preparation and minimizing the risk of precipitation or crystallization, ensuring the stability of detection results.

2. High sensitivity: Breaking through the limit of trace detection

TOOS can still produce significant color changes at low concentrations and is suitable for detecting trace samples. Its molar absorbance reaches 3.92 × 10 ⁴ L/(mol · cm), which is more sensitive to color reaction than traditional chromogenic substrates. It can also accurately detect some trace amounts of test items.

3. High stability: Extend the validity period of the reagent

TOOS has stable chemical properties and can be stored for 12 months at low temperatures. Its color products are not easily faded and are suitable for automated detection systems. The inter batch difference is less than, ensuring consistency in large-scale clinical testing.

Product packaging

Hubei Xindesheng Material Technology Co., Ltd. specializes in the research and production of TOOS and other chromogenic substrates. With rich production experience and professional technical and quality inspection personnel, we can provide excellent technical support and after-sales service. We have established cooperation with many domestic and foreign enterprises and received high praise. We have various online branches selling products, and offline we have large factories, professional technical teams, and advanced production equipment. We welcome users to visit!