TOOS 82692-93-1, a chromogenic substrate, can be used to determine the concentration of uric acid in human serum

In the field of biochemical detection, accurate measurement of various indicators of the human body is of crucial significance for disease diagnosis, health assessment, and disease monitoring. Among them, uric acid, as a key product of purine metabolism in the body, can intuitively reflect the health status of the body at its concentration level. And the chromogenic substrate N-ethyl-N - (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt (TOOS reagent), with its unique properties, has become a powerful "assistant" for determining uric acid concentration in human serum.

1, Uric Acid Measurement: A Key Window for Insight into Health

Uric acid plays an important role in the metabolic process of the human body. It originates from the metabolism of endogenous purine, where adenosine deaminase (ADA) catalyzes the deamination of adenine nucleoside to hypoxanthine nucleoside, which is then metabolized into urea through a series of enzymatic reactions. The normality of uric acid content is directly related to the health status of the body. When uric acid excretion is reduced or purine metabolism is excessively high, it may lead to hyperuricemia, which in turn can cause serious diseases such as gout, causing great pain to patients. On the contrary, insufficient or excessive production of uric acid can lead to hypouricemia, which can also have adverse effects on the body.

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2, TOOS: An Outstanding Representative of the New Trinder's Reagent

TOOS belongs to the new type of Trinder's reagent, which has demonstrated extraordinary performance in biochemical testing. The enzymatic reaction between TOOS and hydrogen peroxide has extremely high sensitivity and specificity, making it an ideal choice for measuring the indicators that can generate hydrogen peroxide in biochemical detection. Among numerous application scenarios, using TOOS to measure uric acid concentration in human serum is one of its typical applications, providing reliable technical support for clinical diagnosis and scientific research.

3, TOOS enzymatic determination of uric acid: the scientific mystery of precise color development

Under the catalytic action of uricase, uric acid undergoes an oxidation reaction with oxygen and water, producing allantoin and hydrogen peroxide. This reaction is a crucial starting step in the entire measurement process. Subsequently, in the presence of peroxidase, the chromogenic substrate TOOS undergoes a coupling reaction with 4-aminoantipyrine (4-AAP), which is oxidized by the hydrogen peroxide generated in the previous step, resulting in the formation of red quinone imine compounds. The color depth of this substance is directly proportional to the amount of color products, which is closely related to the content of uric acid. By measuring the absorbance of the generated substance using a spectrophotometer, the concentration of uric acid in human serum can be accurately calculated based on the proportional relationship between absorbance and uric acid content.

In order to simplify the experimental operation and avoid the tedious process of repeatedly adding reagents, researchers cleverly divided the non reactive reagent combinations into two groups. Reagent 1 mainly includes biological buffer, peroxidase, and chromogenic substrate TOOS; Reagent 2 is mainly composed of uricase, 4-AAP, and sodium azide. During the experiment, simply mix these two sets of reagents in different proportions to easily complete the preparation work before the measurement.

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In addition to TOOS enzyme photometry, there are also methods using chromogenic TOPS or TMB enzyme-linked immunosorbent assay to determine uric acid, which have similar principles. As a professional IVD raw material manufacturer, Hubei Xindesheng Material Technology Co., Ltd. can provide various chromogenic substrate such as TOOS, TOPS, MAOS, etc. If you need them, please feel free to contact me!