Analysis of the Application of Chromogenic Substrate TOPS 40567-80-4 in Serum Uric Acid Content Detection

In the field of clinical biochemical testing, the application of various chromogenic substrates provides crucial support for accurate detection. As one of these substrates, TOPS reagent plays a key role in scenarios such as serum uric acid content testing. This article will conduct a detailed discussion on the characteristics of TOPS, its applicable testing areas, and its specific applications in serum uric acid detection.

1, Basic characteristics of chromogenic substrate TOPS

The chromogenic substrate TOPS, whose full name is N-ethyl-N - (3-sulfopropyl) -3-methylaniline sodium salt, has a certain correlation with common TOOS in molecular structure. The difference between the two is mainly reflected in the sulfopropyl moiety on the single atom connected to the benzene ring, which is one less hydroxyl group than TOOS. This structural difference makes TOPS slightly more stable.

TOPS powder

Meanwhile, TOPS, similar to TOOS, has the characteristic of high water solubility, which enables it to better integrate with other reagents during clinical testing, facilitating smooth subsequent reactions. In addition, TOPS also has the advantage of high detection sensitivity, which can accurately capture subtle changes during the detection process, providing strong guarantees for the accuracy of detection results. These characteristics have also made TOPS widely concerned and applied in the field of clinical biochemical detection.

2, The applicable testing fields of TOPS

TOPS has a wide range of applications and is involved in both kidney function and liver function related tests. In terms of renal function testing, TOPS, like TOOS, can be used for uric acid detection in renal function test kits. However, there are slight differences in the selection of colorants for uric acid detection kits produced by different companies, and some companies use ESPMT as a chromogenic substrate, which actually refers to TOPS.

In addition to uric acid testing, TOPS also has important applications in creatinine testing of renal function series, which can help clinical patients accurately understand their renal function status. In the field of liver function testing, TOPS is commonly used for free fatty acid testing in the liver function series. By detecting the content of free fatty acids, it provides valuable reference for clinical evaluation of patients' liver function and supports doctors' diagnosis and treatment.

3, The specific application of TOPS in serum uric acid detection

TOPS plays an indispensable role in the clinical detection of serum uric acid levels, and the entire detection process must strictly follow standardized procedures to ensure the accuracy of the test results.

Firstly, in the sample collection stage, there are clear requirements that need to be followed before collecting venous blood from the test object. The test subjects need to maintain an empty stomach for 12 hours, usually choosing to have blood collected in the morning. At the same time, they cannot drink alcohol within 12 hours before blood collection. These requirements are to avoid interference from diet and alcohol factors on the test results. During the blood collection process, disinfection of the blood collection tube is also crucial, and strict adherence to disinfection standards is required to prevent sample contamination. In addition, the collected blood samples must ensure that there is no hemolysis, as hemolysis samples can affect the accuracy of the test results. If hemolysis samples occur, they need to be collected again.

After the sample collection is completed, it enters the detection stage, which uses a typical two-step enzymatic reaction to achieve uric acid analysis. The first step is that under the catalytic action of uricase, uric acid in the sample reacts with oxygen and water, and is completely oxidized to produce allantoin, carbon dioxide, and hydrogen peroxide (H ₂ O ₂). In the second step, the generated hydrogen peroxide reacts with TOPS and 4-aminoantipyrine (4-AAP) under the catalysis of peroxidase (POD), during which TOPS is oxidized to form color products.

Finally, measure the absorbance of the colorimetric product using a spectrophotometer. Due to the proportional relationship between absorbance intensity and uric acid content in the sample, the specific content of serum uric acid in the sample can be determined by analyzing and calculating absorbance, thus completing the detection of serum uric acid content and providing accurate data support for clinical diagnosis and treatment.

Product packaging

Hubei Xindesheng Material Technology Co., Ltd., as a high-quality developer and manufacturer of chromogenic substrates, can provide high-quality diagnostic reagent raw materials for in vitro diagnostic kit manufacturers. If you are also interested in TOPS and other new trinder's reagents, please click on the official website to learn more details or contact me!