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|Appearance:||White Crystal Powder||Molecular Weight:||279.33|
|Molecular Formula:||C12H18NNaO3S||CAS #:||40567-80-4|
|PH Range Of Solution:||5.5-9.5||Molar Absorbance:||3.74*10^4|
TOPS Trinder Reagent,
Cas 40567-80-4 TOPS,
Three advantages of Desheng chromogen substrate TOPS
Keywords: chromogen substrate, TOPS, N-ethyl-N-(3-sulfopropyl)-3-methylaniline sodium salt Desheng
The full name of TOPS in Chinese is N-ethyl-N-(3-sulfopropyl)-3-methylaniline sodium salt, its CAS number is 40567-80-4, the molecular formula is c12h18nnao3s·h2o, and the molecular weight is 297.34. It can be applied to enzymes. A water-soluble reagent for the photometric determination of hydrogen peroxide, which is widely used in diagnostic testing and biochemical tests.
Three advantages of Desun products TOPS:
1. Desheng has always been adhering to the company culture of virtue-oriented, honesty first, quality first, and leading technology. It specializes in the research and development, production and sales of blood collection tube additives, in vitro diagnostic reagents, buffers, and luminescent substrates. And there is a dedicated research and development department, so Desheng products are supplied by TOPS manufacturers, and the price is favorable and the price difference is not earned.
2. Desheng products are ready-to-use and can be packaged according to customer needs. And the sub-assembly process is strict, and it is carried out in a professional sub-assembly table. The sub-assembly table is kept clean. The sub-packaging personnel must wear disposable gloves for sub-packaging. The clothing requirements of the staff are even stricter.
3. Desheng delivers fast. Orders are delivered on the same day (before four o'clock). The staff has a customer-oriented philosophy. Once the company had a customer with a very large order but the packaging was cumbersome. The company guaranteed that the customer’s order was on time Delivery, all staff help to sub-package, to ensure the delivery of the product.
Application of TOPS:
Biochemical research. Enzymatic spectrophotometric determination of water-soluble reagents for hydrogen peroxide. The new Trinder"s reagent is a highly water-soluble aniline derivative, which is widely used in diagnostic testing and biochemical tests. It has several advantages over conventional chromogenic reagents in the colorimetric determination of hydrogen peroxide activity. The new Trinder"s reagent is sufficiently stable , It can be used in both solution and test assembly line detection system. In the presence of hydrogen peroxide and peroxidase, the new Trinder's reagent is in the oxidative coupling reaction process with 4-aminoantipyrine (4-AA) or 3-methylbenzothiazole sulfone hydrazone (MBTH) In, a very stable purple or blue dye is formed. The molar absorbance of the dye coupled with MBTH is 1.5-2 times higher than that of the dye coupled with 4-AA; however, the 4-AA solution is more stable than the MBTH solution. The substrate is oxidized by it. Enzymatic oxidation produces hydrogen peroxide. The concentration of hydrogen peroxide corresponds to the concentration of the substrate. Therefore, the amount of substrate can be determined by the color development of the oxidative coupling reaction. Glucose, alcohol, acyl-CoA and cholesterol can be used for Detect those substrates coupled with the new Trinder's reagent and 4-AA. There are 10 new Trinder’s reagents. Among the new Trinder’s reagents, TOOS is the most commonly used. However, for specific substrates, testing different kinds of new Trinder's reagents is necessary to develop the best detection system.
TOPS is stored at 0-5°C, protected from light and moisture, with a purity of >99% and good water solubility. The process is stable, and the appearance of the product can be guaranteed to be pure white crystalline powder. Desheng is a veteran blood testing reagent company with 14 years of R&D and production experience, and has in-depth research on the chromogen substrate Trinder’s reagent.
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