As an important chemiluminescent reagent, acridine ester plays an important role in chemiluminescence immunoassay. Compared with other luminescence systems, it has its special advantages: for luminescent markers, the label of acridine esters is relatively Simple, stable luminescence of the marker, long validity period of the kit, and relatively low cost; and the luminescence is flash type, the luminescence is fast and concentrated, and the intensity is high, which is convenient to realize rapid detection, and the detection sensitivity and precision are high. The requirements are relatively simple and easy to realize fully automated operation; in addition, the acridinium ester luminescence system has few interference factors, extremely low background, and high signal-to-noise ratio. Based on these advantages of the acridine ester luminescence system, the research and development of a chemiluminescence substrate solution suitable for the acridinium ester chemiluminescence system is of very positive significance.
Method for preparing acridine ester chemiluminescent substrate solution:
A chemiluminescence substrate solution suitable for acridine ester luminescence system, buffer A and buffer B separately stored before use:
Buffer A: inorganic acid and oxidant, and the pH of buffer A is less than 2; the concentration of acid is 0.03-0.10M, and the mass concentration of oxidant is 0.3-1.2wt%;
Buffer B: inorganic base and enhancer, and the pH of buffer B>13; the concentration of base is 0.2-0.65M, and the mass concentration of enhancer is 0.2-2wt%. The volume ratio of buffer A and buffer B is 2:3-3:2.
(1) Preparation of Buffer A: Put 4.2L purified water, 41.7mL 37% hydrochloric acid and 50.2g carbamide peroxide in a 5L wide-mouth glass container that is protected from light, add purified water to make the volume to 5L, stir and mix, and filter to obtain Buffer A;
The pH of the preparation buffer A is 1.0, where the concentration of hydrochloric acid is 0.10M, and the mass concentration of carbamide peroxide is 1.0 wt%;
(2) Preparation of buffer B: Add 4L purified water and 100.6g octaalkyltrimethylammonium chloride to a 5L wide-mouth glass container in turn, stir until the solid is completely dissolved, add 80.0g sodium hydroxide, and stir until complete After dissolving, add purified water to make the volume to 5L, and filter to obtain buffer B;
The pH of the preparation buffer B was 13.3, the concentration of sodium hydroxide: 0.40M; the concentration of octaalkyltrimethylammonium chloride: 2.0wt%.
As a manufacturer, Desheng has been researching and developing in the field of chemiluminescence reagents for 15 years. The acridinium ester series developed by it has the advantages of good stability, high sensitivity, wide detection range and low cost.
Contact Person: Miss. Ankiwang