DNA nucleic acid electrophoresis is an important step in nucleic acid PCR, separation and purification, nucleic acid detection and other tests. The agarose gel electrophoresis kit is an electrophoresis kit product developed to facilitate nucleic acid electrophoresis. Compared with traditional gel electrophoresis has many advantages.
Agarose gel, electrophoresis fluid and loading buffer
Electrophoresis refers to the movement of charged particles toward an electrode opposite to their electrical properties under the action of an electric field. Under certain conditions, the moving rate (mobility) of charged particles is fixed. In DNA electrophoresis or Southern Blot blot hybridization, charged particles refer to nucleic acid DNA, and different DNAs have different mobilities and thus separate from each other. Since nucleic acid particles are very sensitive to pH, a buffer must be added to the electrophoresis solution to nucleic acids. The buffer components are contained in agarose gel, TAE or TBE electrophoresis solution, loading buffer.
Generally, agarose gel electrophoresis needs to go through the following steps: preparation of agarose electrophoresis gel, preparation of electrophoresis solution, spotting, electrophoresis, and cleaning of electrophoresis tank. Among them, the longest time is the preparation of agarose gel. It needs to prepare a mixing solution, weigh agarose, melt in a microwave oven, cool the solution to 60 degrees, pour the gel to cool, and clean the equipment. The process is very cumbersome, and repeated in large quantities, it takes 1 ~ 1.5 hours. The agarose gel electrophoresis kit is different:
1. There is no need to purchase reagents such as agarose, nucleic acid dye, electrophoresis solution and loading buffer.
2. Eliminate the cumbersomeness of making gel, and the pre-made gel is pre-stained with nucleic acid dyes, no need for electrophoresis solution staining or post-staining. Simple and convenient, ready to use. No need to add nucleic acid dye in DNA samples or electrophoresis solution when using pre-made gel, reducing the waste of nucleic acid dyes, and the extremely low toxicity of pre-stained gels is much safer for operators than directly use nucleic acid dyes.
3. The kit is specially equipped with high-pressure fast electrophoresis solution. If your nucleic acid sample fragment is below 2000bp, you can control the speed of electrophoresis at any time and adjust the voltage. The general mini gel can be completed in 5-10 minutes at the fastest; Marker or Nucleic acid samples have many bands and long fragments (nucleic acid sample fragments are larger than 2000bp), which can be adjusted to a suitable low voltage, or you can still use your original low-voltage electrophoresis conditions.
4. The electrophoresis of this product does not affect the subsequent Southern hybridization, and the obtained DNA fragments are subjected to gel recovery, and the subsequent DNA ligation and other reactions are not affected.
In short, compared with the traditional nucleic acid electrophoresis method, the agarose precast gel electrophoresis kit has the advantages of saving time,saving labor , high pressure, fast, and saving cost . It is a product strongly recommended by Desheng. Of course, there are TAE, TBE electrophoresis kits or Tris buffer or other buffers can also contact our company.
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