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|Molecular Weight:||121.135||Molecular Formula:||C4H11NO3|
|Appearance:||White Crystal Powder||Storage Conditions:||Room Temperature, Light-proof And Moisture-proof|
anticoagulant citrate dextrose solution,
n cyclohexyl 3 aminopropanesulfonic acid
TRIS buffer solution|Tris((hydroxymethyl) aminomethane|Tris Base CAS 77-86-1
Tris (hydroxymethyl) aminomethane
Room temperature, light-proof and moisture-proof
White crystal powder
Buffer preparation in biochemical and molecular biology experiments; pharmaceutical intermediates; preparation of surfactants and vulcanization accelerators; preparation of water-soluble polymers containing Tris structural units as paint dispersants; preparation of indoor formaldehyde adsorption materials, etc.
The purity (> 99%) is water-soluble and the process is stable.
Tris is often used as biological buffer, and its pH value is 6.8, 7.4, 8.0, 8.8. Its structure formula, pH value changes greatly with temperature. Generally speaking, the pH value decreases by 0.03 for each degree of temperature rise.
Tris is widely used in the preparation of buffers in biochemistry and molecular biology experiments. For example, both TAE and tbe buffers (used for nucleic acid dissolution) commonly used in biochemical experiments need to use Tris. Because it contains amino group, it can condense with aldehyde.
Tris is a weak base, and its PKA is 8.1 at room temperature (25 ℃; according to the buffer theory, the effective buffer range of Tris buffer is between pH7.0 and 9.2.
The pH value of Tris base aqueous solution is about 10.5. Generally, hydrochloric acid is added to adjust the pH value to the required value to obtain the buffer solution of the pH value. However, the effect of temperature on the pKa of Tris should also be noted.
Configuration of Tris buffer solution
|PH value required(25℃)||7.10||7.20||7.30||7.40||7.50||7.60||7.70||7.80||7.90||8.00||8.10||8.20||8.30||8.40||8.50||8.60||8.70||8.80||8.90|
|0.1mol/L HCI volume||45.7||44.7||43.4||42||40.3||38.5||36.6||34.5||32||29.2||26.2||22.9||19.9||17.2||14.7||12.4||10.3||8.5||7|
Tris is a weak base, and its PKA is 8.1 at 25 ℃; according to buffering theory, the effective buffering range of Tris buffer is between pH7.0 and 9.2. The pH value of Tris base aqueous solution is about 10.5. Generally, hydrochloric acid is added to adjust the pH value to the required value to obtain the buffer solution of the pH value. However, the effect of temperature on the pKa of Tris should also be noted.
Hubei New Desheng Materials Technology Co., Ltd. is specialized in producing Tris products. We have an excellent R&D team, with super-high requirements for product quality. The monthly output is about 100t, and enterprises or individuals who need, please let me know in advance for a special stock. Looking forword to your further consultation!
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