Tris Buffer CAS 77-86-1 Tris Hydroxymethyl Aminomethane Buffer Solutions

Buffer solutions;Tris Buffer;CAS 77-86-1;tris hydroxymethyl aminomethane

The PH range and advantages and disadvantages of Tris buffer

The buffer solution is a kind of solution that can resist the influence of a small amount of strong acid and alkali from the outside and maintain the pH value of the system basically unchanged. It has extremely important significance in the research of biochemistry. Hydroxymethylaminomethane is a widely used buffer in biochemical research. Let's start with its PH value.

Tris White Powder

Tris buffer pH range

Tris buffer is a widely used buffer in biochemical research. Its commonly used effective pH range is in the "neutral" range, such as:

Tris-HCl buffer: pH=7.5～8.5

Tris-phosphate buffer: pH=5.0～9.0

In addition to TRIS-hcl, TRIS also has a variety of derivatization buffers:

TBS=Tris-HCl+NaCl+KCl is often used to clean the Western blotting membrane in immunostained tissue or Western blotting;

TBST=Tris-HCl+NaCl+tween20, a membrane buffer commonly used in Western Blotting;

TE=Tris-HCl+EDTA, has a protective effect on DNA bases, and is often used for DNA stabilization and storage;

TAE = Tris base + acetic acid + EDTA, is a buffer system widely used in short DNA electrophoresis;

TBE = three bases + boric acid + EDTA, suitable for long-term DNA electrophoresis, and has a good separation effect on small fragments.

Configuration of Tris buffer solution

Advantages of Tris buffer

① Because the Tris base is highly alkaline, this buffer system can be used to prepare buffers with a wide range of pH values ​​ranging from acidic to alkaline;

② It has little interference to the biochemical process, and does not precipitate with calcium, magnesium ions and heavy metal ions.

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Disadvantages of Tris buffer

① The pH of the buffer is greatly affected by the concentration of the solution, the buffer is diluted ten times, and the pH change is greater than 0.1;

② The temperature effect is large, and the temperature change has a great influence on the pH value of the buffer solution. △pKa/℃＝-0.031, for example: the pH of the buffer solution at 4℃=8.4, then the pH=7.4 at 37℃, so it must be Prepare at the temperature of use. Tris-HCl buffer prepared at room temperature cannot be used at 0℃～4℃;

③ It is easy to absorb CO2 in the air, so the prepared buffer should be tightly sealed;

④ This buffer has a certain interference effect on some pH electrodes, so use an electrode compatible with Tris solution.

It can be seen that adding a small amount of strong acid or strong base to the buffer solution does not change the pH value of the solution much, but if the amount of acid and alkali is large, the buffer solution loses its buffering effect. This shows that its buffer capacity is limited. The buffer capacity of the buffer solution is related to the concentration of the components that make up the buffer solution. After 15 years of independent R&D and production, Desun Technology has been widely recognized and used by customers in the Chinese local market. Many well-known domestic manufacturers have been choosing and using Desheng products for many years. We can also customize buffers with different pH values ​​according to customer needs.