Application and synthesis of TAPS Tris(hydroxymethyl)methylaminopropanesulfonate

Tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid or TAPS, CAS: 29915-38-6, is a zwitterionic buffer, widely used in the field of biochemistry and molecular biology, usually white crystals or Powder is a Good's biological buffer mainly produced by the company.

In clinical diagnostic biochemical testing, TAPS is mainly used as a biological buffer. Its molecular structure contains a sulfonic acid group and an amino group substituted by a polyhydric alcohol, the sulfonic acid group is weakly acidic, and the amino group is weakly basic, so as to maintain the pH value of the reaction system, the buffer range is 7.7-9.1; Good, the solubility can reach 50g per 100g of water; therefore, it is often used in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits.

Tris(hydroxymethyl)methylaminopropanesulfonic acid TAPS

In addition to the kit, TAPS is also used to protect oxyhemoglobin during freeze-drying, as a protective agent to prevent the oxidation of hemoglobin to methemoglobin. This is very important, because traditional blood transfusions have many drawbacks, such as blood-borne infectious diseases, complicated blood type matching, and viral infections. Hemoglobin oxygen carriers can be used as good blood substitutes. However, hemoglobin is easily oxidized to methemoglobin without oxygen carrying capacity during the freeze-drying process, so it is necessary to add a protective agent to prevent hemoglobin degeneration, TAPS is one of the important components.

At present, a domestic TAPS synthesis method is: Tris methylaminomethane Tris and 1,3-propane sultone (1,3-PS) in n-butanol solvent, Tris amino nitrogen atom and Hydrogen atoms are added to the carbon and oxygen in which propane sultone is broken and ring-opened to form TAPS. In this reaction, n-butanol can be recycled to improve product yield and purity.

TAPS, a buffer used in biochemical testing and molecular diagnostics, has very high requirements on reagent purity and impurity ion content. Desheng Technology has done a lot of research and improvement in this area, and many biological buffers produced by the company have obtained a large number of Recognized by biochemical testing companies and medical device companies.