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Biological buffer TAPS buffer solutions ,CAS29915-38-6,Purity > 99%

Product Details

Place of Origin: China

Brand Name: De Sheng

Certification: ISO9001

Model Number: TAPS

Payment & Shipping Terms

Minimum Order Quantity: 500g

Price: Negotiable

Packaging Details: Plastic bottle

Delivery Time: 1-3 days

Payment Terms: L/C, D/A, D/P, T/T, Western Union, MoneyGram

Supply Ability: 1000kg/month

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Specifications
Highlight:

TAPS buffer

,

29915-38-6

,

Biological buffer

Name:
TAPS
Appearance:
White Powder
Purity:
> 99%
CAS#:
29915-38-6
Molecular Weight:
243.28
Molecular Formula:
C7H17NO6S
Name:
TAPS
Appearance:
White Powder
Purity:
> 99%
CAS#:
29915-38-6
Molecular Weight:
243.28
Molecular Formula:
C7H17NO6S
Description
Biological buffer TAPS buffer solutions ,CAS29915-38-6,Purity > 99%

 

 

Name

 

3-[Tris(hydroxymethyl)methylamino]-1-propanesulfonic acid

 

Abbreviation

 

TAPS buffer

 

CAS#

 

29915-38-6

 

Molecular weight

 

243.28

 

Molecular formula

 

C7H17NO6S

 

Storage Conditions

 

Room temperature, light-proof and moisture-proof

 

Biological buffer TAPS buffer solutions ,CAS29915-38-6,Purity > 99% 0

TAPS buffer

 

Use

 

As a buffer system commonly used in DNA screening system, it can also be used as a buffer component of RNA samples. It is suitable for the study of electron transfer and phosphorylation of thin-layer chloroplast preparation. It can protect oxyhemoglobin from oxidizing to methemoglobin during freeze-drying process, and can also be used as a background electrolyte for protein microanalysis by capillary zone electrophoresis.

 

Advantage

 

The purity (> 99%) is water-soluble, the process is stable, and the appearance of the product can be guaranteed to be pure white crystal powder.

 

Biological buffer for separation of dyes in chromatography

 

Chromatography is a laboratory technique, which is usually used to separate and purify proteins. In this process, the separation ability of the system is directly related to the change of pH. For example, if the pH value of the mobile phase (solvent) is close to pKa, a small change in the pH value will seriously affect the retention rate, thus leading to separation. Due to the retention of ionizable compounds.The mobile phase pH is particularly sensitive, so a buffer should be added to the system to control this variable.

 

Based on the most relevant literature on chromatography, Desheng's researchers found that although Tris and MES are generally considered to be the best choice, other buffers include taps, which have also been used in cation exchange chromatography, anion exchange chromatography, high performance liquid chromatography (HPLC) and other similar technologies.

 

One of the most suitable biological buffers for cell culture

 

The most important characteristics of good's buffer.One is that they are not toxic to cells. Therefore, these chemicals are widely used in cell culture to keep the experiment,The pH value of is under control. Due to their special functions, many good's Buffers / biological buffers.It is considered to be an ideal choice for cell separation, cell culture, enzyme analysis and many other biochemical applications.

 

Desheng's researchers found that protonated sulfamate compounds directly inhibited the activity of connexin channels.In good'sph buffer (MES (4-morpholine ethane sulfonic acid), HEPES and taps (3 - {[2-hydroxy-1,1-In bis (hydroxymethyl) ethyl] amino] - 1), the pH dependent activity of the linker channel demonstrates this.Propane sulfonic acid), they have a common sulfamate moiety, and there is no sulfamate.Part (of the pH buffer does not have pH dependent channel activity.Therefore, taps is more used for cell culture Experiments on flagellate algae in the substrate.

 

Preparation method of taps solution (about 1-2l)

 

(1) Prepare 0.1M solution (a): taps 24.328g/deionized water 1000ml

 

(2) Prepare 0.1M NaOH solution (b): NaOH 4G / deionized water 1000ml

 

pH 4.6

1,000ml(A)+0ml(B) (A):(B)=5:0

pH 7.8

1,000ml(A)+200ml(B) (A):(B)=5:1

pH 8.3

1,000ml(A)+400ml(B) (A):(B)=5:2

pH 8.6

1,000ml(A)+600ml(B) (A):(B)=5:3

pH 9.0

1,000ml(A)+800ml(B) (A):(B)=5:4

* temperature 20 degrees.

* use a pH meter if you need to adjust to a specific pH.

* do not want to join Na, please use KOH.

 

Biological buffer TAPS buffer solutions ,CAS29915-38-6,Purity > 99% 1

Product packaging

 

Hubei new Desheng Material Technology Co., Ltd. is specialized in the production of biological buffer taps products, and has 15 years of production experience. We have a professional R & D team, which can solve the follow-up problems for you. We can also customize the indicators of the products, please contact us, welcome your consultation!

 

 

 

 

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