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Good's Buffer
, CAPS buffer
, 1135-40-6
Good Buffer Solutions CAPS High Purity> 99% White crystal powder CAS1135-40-6
Brief introduction
| Full Name |
N-Cyclohexyl-3-aminopropanesulfonic acid |
Molecular weight |
221.32 |
| Abbreviation |
CAPS |
Molecular formula |
C9H19NO3S |
| CAS No |
1135-40-6 |
ENIECS |
214-492-1 |
| Appearance |
White crystal powder |
Purity |
≥99% |
Good's buffer, which is zwitterionic buffer. This type of buffer is generally a sulfamic acid-based organic compound. After the solution is formulated, the pH is relatively stable and does not react with various enzymes and chemical reagents. It is mainly used in biology and medicine.
CAPS buffer
Good’s buffer has the following general characteristics
(1) The solubility in water is high;
(2) acid and alkali dissociation constant (pKa value) is stable, generally in 6~8, the ability to release protons is stable, and little affected by the ion concentration, solution composition and temperature;
(3) has a low cell membrane permeability, it is not easy to penetrate the biofilm;
(4) can chelate with metal ions in low molar mass, but not generate precipitation with metal ions;
(5) Chemical stability is high;
(6) the light absorption in the ultraviolet and visible wavelength ranges is small;
(7) the possibility of salting out with the organic macromolecules in the organism is small;
(8) the high purity salt is easy to obtain; (9) does not participate in the biochemical reaction process, does not interfere with the biochemical reaction process.
At present, the commonly used Good's buffers are shown in the following table, in which the concentration of buffer and sodium hydroxide is 0.1mol/L.
Application of CAPS
At present, the PVD transmembrane of protein has not used Tris-gly, SDS, 20% methanol buffer, because there is glycine in the buffer, so it is difficult to distinguish whether glycine is brought in by Buffer or the protein itself when sequencing. Now the transmembrane buffer used for sequencing is 10 mM/1 CAPS 10% methanol, and the methanol concentration range in CAPS imprint buffer is 0-20%. In general, the buffer with high methanol concentration is paired. The transfer efficiency of low molecular weight proteins is good, while the buffer with low or no methanol concentration is beneficial to the transfer of high molecular weight proteins.
Advantage of CAPS
The purity (> 99%) is water-soluble, the process is stable, and the appearance of the product can be guaranteed to be pure white crystal powder.
Product packaging
Preparation of CAPS
prepare 0.1mol/L buffer solution with the volumetric flask. During the configuration process, the CAPS buffer needs high temperature dehydration, and cooling in the dryer; preparation of 0.1mol/L sodium hydroxide solution. According to the formula in the above table, the corresponding pH buffer can be obtained, sodium hydroxide is mainly used to adjust the pH.
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