MOPS (3-morpholinopropanesulfonic acid) is a zwitterionic buffer, namely Good'sBuffers, a series of N-substituted aminosulfonic acids, have good pH stability, high polarity, are inert to a variety of chemical reagents and enzymes, do not participate in and do not interfere with the biochemical reaction process, and have no inhibitory effect on enzymatic chemical reactions, so they can be specially used in the research of organelles and highly variable, pH-sensitive proteins and enzymes.
||3-(4-morpholino)propane Sulfonic Acid; 3-(morpholin-4-yl)propane-1-sulfonic Acid; 4-(morpholinopropane Sulfonic Acid); 4-morpholinepropanesulfonic Acid;
||1000 g/L (20 ºC) in water
In addition, MOPS has the following characteristics common to Good's buffer system:
1. The pKa value is between 6 and 8;
2. High solubility in water;
3. It is not easy to penetrate biofilm;
4. Small salt effect;
5. Ion concentration, solution composition and temperature have little effect on dissociation;
6. No complex or precipitation with metal ions;
7. The buffer is chemically stable;
8. Absorption in the ultraviolet region of 0.1 mol/L aqueous solution, the smaller the absorbance at 260 and 280 nm wavelength, the better;
9. High purity salt can be easily prepared.
MOPS BufferPKa: 7.2PH range: 6.5-7.9, commonly used in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits.It is suitable for the study of electron transfer and phosphorylation in thin-layer preparation of chloroplasts; it can be prepared into a variety of agar media and used as a non-toxic buffer in the limit medium for the culture of Streptomyces coryniformis and the production of cephalosporins; it can be used as an electrolyte system component in two-dimensional gel electrophoresis medium electrofocusing electrophoresis (IEF); it can also be used in Northern hybridization for the isolation and rotation of RNA.Buffer for membrane.
If the amount of each use is small, it can be properly sub-packed before use; the buffer is easy to turn yellow after light, light yellow can be used completely, too dark color should not be used.Attention should be paid during the use. For safety and health, please wear experimental clothes and disposable gloves. If you accidentally contact your eyes, wash them with plenty of water immediately and seek medical treatment immediately.
Desheng is a diagnostic reagent raw material manufacturer with many years of research and development and production experience. The MOPS products produced have a high purity of 99%, good water solubility, stable production process, and strict quality management system to ensure that customers provide high-quality products and services. In addition to buffer products such as MOPS, TRIS, CAPS, TAPS, etc., it also produces blood collection additives, chemiluminescent reagents, chromogenic substrates.Many kinds of products, please call for more information!