Application of biological buffer Tris in Nucleic Acid Gel Electrophoresis

Trimethylolminomethane-Tris is a kind of buffer which is widely used in the field of biochemistry. Its CAS number is 77-86-1. It has the advantages of stable property, not participating in biochemical process and strong buffering ability. It is widely used in the detection of protein and nucleic acid.

Due to the wide application of Tris, some details need to be noted. Although Tris buffer has strong buffering capacity, it needs to be used carefully when the dilution is too large or the volume of reaction system changes greatly. When the dilution is ten times, the pH change is greater than 0.1, and the pH change of phosphate buffer is less than 0.1.

When preparing nucleic acid agarose gel electrophoresis, the first preparation work is to prepare gel. The quality of agarose gel directly influences electrophoresis efficiency and efficiency. This process takes a long time and saves time to purchase the prepared gel directly.

TAE agarose nucleic acid electrophoresis gel

After the electrophoretic solution is prepared, it can be put into the electrophoresis tank, start sampling, and then turn on the power supply to start electrophoresis. Using TAE/TBE, it generally takes 20-30 minutes to complete electrophoresis, and the voltage is recommended not to exceed 200V. The voltage of TAE is relatively higher than that of tbe electrophoretic solution. The higher the voltage, the faster the electrophoresis rate, but the corresponding resolution will be lower.

The conductivity of tbe is higher than that of TAE. Therefore, compared with TAE, tbe is less likely to cause overheating in the electrophoresis tank, so tbe is recommended for long-term electrophoresis. Boric acid is an inhibitor of enzyme, so if you need to separate electrophoresis DNA for enzyme cutting reaction, TAE is recommended as electrophoresis buffer solution. TAE is better for the separation of longer fragments, and tbe is suitable for fragments less than 300 BP. TAE is more suitable for the recovery of DNA fragments.

Tris, like bicine, is a biological buffer developed and produced by Desheng. In addition, it also has rich production experience in EDTA, virus transportation medium and nucleic acid extraction solution associated with it. Looking forwards to your further consulation.