The chromogenic substrate TOPS, the full name of N-ethyl-N- (3-sulfopropyl) -3-methylaniline sodium salt, is similar to TOOS, the difference in molecular structure is that the sulfopropyl group on the single atom connected to the benzene ring With a hydroxyl group, the stability is slightly stronger, and all have the characteristics of high water solubility and high detection sensitivity.
Like TOOS, TOPS can also be used for uric acid detection in kidney function kits. The chromogen used by uric acid detection kits of different companies is slightly different (the chromogenic substrate ESPMT used by some companies refers to TOPS). In addition, TOPS is also commonly used for liver function series free fatty acid detection and renal function series creatinine detection. The following is an example of the principle of uric acid detection.
During clinical testing, first collect venous blood from the test subject. The subject should be fasting for 12 hours. It is best not to drink alcohol in the morning and 12 hours. The blood collection tube should be disinfected. The blood sample is not hemolytic. The detection of uric acid is to first catalyze the complete oxidation of uric acid with oxygen and water to allantoin, carbon dioxide and hydrogen peroxide H2O2. H2O2 reacts with TOPS and 4-AAP under the catalysis of POD. TOPS is oxidized into a chromogenic product, and the absorbance is measured with a spectrophotometer. The intensity of the absorbance is proportional to the uric acid content, so that uric acid analysis is performed. This is a typical biochemical detection method using two-step enzymatic reaction to measure.
Desheng Technology has more than ten years of experience in R&D, production and sales in the field of diagnostic raw materials, especially in the development and improvement of new Trinder's reagents in the aniline sodium salt derivative series. It performs functional groups on the basis of traditional chromogenic substrates. Replacement of the increase and decrease, continue to make optimization and performance improvement for the needs of clinical testing!
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