Biochemical Testing Kits with New Trinder's Reagents
The new Trinder's reagents are highly water-soluble aniline derivatives that are widely used in diagnostic and biochemical tests. The principle is that the tested substances react with enzyme,produce hydrogen peroxide (H2O2) which synthesized red Quinoneimine compound with the help of 4-aminotipyrine (4-AAP) and peroxidase (POD).Initially, phenol was used as a chromogen agent, and later, there were many kinds of compounds to replace phenol, which greatly improved the sensitivity of the reaction. There are many principles of in vitro diagnostic reagents or kits. For example: Enzyme-Linked ImmunoSorbent Assay (ELISA), enzymatic method, colloidal gold method, western blotting method, etc. Each methods can be applied to the detection of multiple substances. For example, based on the principle of Trinder reaction (also called enzymatic method), it can be used for the detection of uric acid, creatinine, blood sugar, cholesterol, triglyceride, etc.
There are several advantages over conventional chromogenic reagents in the colorimetric determination of hydrogen peroxide activity. The new Trinder's reagents are stable enough to be used in both solution and experimental pipeline detection systems. With the help of hydrogen peroxide and peroxidase in oxidative coupling reaction, the new Trinder's reagent reacts with 4-aminoantipyrine (4-AA) or 3-methylbenzothiazole sulfonehydrazone (MBTH),and forms very stable purple or blue dyes. The molar absorbance of the dye coupled with MBTH was 1.5-2 times higher than that coupled with 4-AA.However, the solution of 4-AA was more stable than the MBTH.The substrate is oxidized by oxidase and produce hydrogen peroxide. The hydrogen peroxide concentration corresponds to the substrate concentration. Therefore, the amount of substrate can be determined by the color of the oxidative coupling reaction. Glucose, alcohol, acyl-CoA and cholesterol can be used to detect those substrates coupled by the new Trinder's reagent and 4-AA.
There are 10 new Trinder's reagents available in Desheng. TOOS is the most commonly used among them. However, for a specific substrate, testing different types of Trinder's reagents is necessary to develop the optimal detection system.For more information,welcome to inquire http://www.whdsbio.cn/product/13.html
