TAPS, the full name N-tris (hydroxymethyl) methyl-3-aminopropanesulfonic acid, CAS No. 29915-38-6; molecular weight 243.28; molecular formula: C7H17NO6S; it is white crystalline powder, soluble in water and other organic solvents , pH buffer range 7.7-9.1. Zwitterionic buffers for biochemical research and molecular biology are currently being used more and more widely in biochemistry, chemistry and medicine. It can be used as bio-buffering agents in biochemical diagnostic kits and DNA / RNA extraction kits. And high-end chemical reagents such as PCR diagnostic kits, cell culture must maintain strict pH values, and as a chelating agent for certain metal ions such as erbium, neodymium, etc., suitable for capillary electrophoresis analysis of DNA and DNA dye complexes , Can inhibit the activity of connexin pipeline, use TAPS physiological buffer in various carbonic anhydrase activities, pKa = 8.4.
There are few reports on the synthesis process of TAPS. In 2010, Chen Gui'e and others studied the synthesis method of TAPS in detail. They use trishydroxymethylaminomethane (Tris) and 1,3-propane sultone (1,3-PS) as raw materials to make them react in alcoholic solvents. Specific operation: Weigh equimolar amount of Tris And 1,3-PS, and take an appropriate amount of alcohol solvent into a 250ml three-necked flask with condensing and refluxing, heating with stirring and refluxing; after a period of reaction, stop the reaction; at room temperature, after the reaction After the solution was allowed to stand and cooled to room temperature, it was suction filtered under reduced pressure. The TAPS crude filter cake was washed 2 to 3 times with an appropriate amount of absolute ethanol. The TAPS filter cake was taken out and placed in a vacuum drying oven to dry. stand-by.
In the above synthesis process, the reaction time, different alcohol solvents and the recycling of the reaction solvent have a certain effect on the TAPS yield and purity. The experimental results show that the reaction time is controlled at 6 hours, and the yield is the highest, reaching 70.3%. Continue to extend the reaction time, the yield decreases slightly; for the ethanol, n-propanol, isopropanol and n-butanol reaction solvents investigated, n-butanol The yield and purity of TAPS as the reaction solvent are the highest, 70.3% and 94.36%, respectively; in the reaction process, the recycling of the reaction solvent is beneficial to improve the yield and purity of TAPS.
Desheng has successfully carried out synthesis experiments based on the results of previous research and development. Now it has accumulated many years of production experience. The production of TAPS standards is high. Now the specifications of Desheng TAPS products are shown as follows:
|Appearance||White crystalline powder|
|Water insoluble matter||≤0.01%|
|Loss on drying||≤1.0%|
|pH(1% water solution)||4.4-6.0|
|Absorbance(260nm 0.1Mwater solution)||≤0.04|
|Absorbance(280nm 0.1Mwater solution)||≤0.02|
The Warning and prevention:
◇ When impurities, foreign matters, abnormal odors, colors, severe moisture absorption and the expiration date are exceeded, the use is prohibited.
◇ Do not continue to use when turbidity appears.
◇ This product is a chemical reagent with strong corrosive properties, fasting, and direct touching by hand are prohibited.
◇ When spilled on the skin, rinse with plenty of water.
◇ If it splashes into the eyes, it can be immediately rinsed with a large amount of water. In an emergency, it should be sent to the hospital for treatment immediately.
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