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Name: | TAPS | Appearance: | White Powder |
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Purity: | > 99% | CAS#: | 29915-38-6 |
Molecular Weight: | 243.28 | Molecular Formula: | C7H17NO6S |
High Light: | anticoagulant citrate dextrose solution,n cyclohexyl 3 aminopropanesulfonic acid |
TAPS CAS 29915-38-6 3-[Tris(Hydroxymethyl)Methylamino]-1-Propanesulfonic Acid
Name
3-[Tris(hydroxymethyl)methylamino]-1-propanesulfonic acid
Abbreviation
TAPS
CAS#
29915-38-6
Molecular weight
243.28
Molecular formula
C7H17NO6S
Storage Conditions
Room temperature, light-proof and moisture-proof
Use
As a buffer system commonly used in DNA screening system, it can also be used as a buffer component of RNA samples. It is suitable for the study of electron transfer and phosphorylation of thin-layer chloroplast preparation. It can protect oxyhemoglobin from oxidizing to methemoglobin during freeze-drying process, and can also be used as a background electrolyte for protein microanalysis by capillary zone electrophoresis.
Advantage
The purity (> 99%) is water-soluble, the process is stable, and the appearance of the product can be guaranteed to be pure white crystal powder.
Biological buffer for separation of dyes in chromatography
Chromatography is a laboratory technique, which is usually used to separate and purify proteins. In this process, the separation ability of the system is directly related to the change of pH. For example, if the pH value of the mobile phase (solvent) is close to pKa, a small change in the pH value will seriously affect the retention rate, thus leading to separation. Due to the retention of ionizable compounds.The mobile phase pH is particularly sensitive, so a buffer should be added to the system to control this variable.
Based on the most relevant literature on chromatography, Desheng's researchers found that although Tris and MES are generally considered to be the best choice, other buffers include taps, which have also been used in cation exchange chromatography, anion exchange chromatography, high performance liquid chromatography (HPLC) and other similar technologies.
One of the most suitable biological buffers for cell culture
The most important characteristics of good's buffer.One is that they are not toxic to cells. Therefore, these chemicals are widely used in cell culture to keep the experiment,The pH value of is under control. Due to their special functions, many good's Buffers / biological buffers.It is considered to be an ideal choice for cell separation, cell culture, enzyme analysis and many other biochemical applications.
Desheng's researchers found that protonated sulfamate compounds directly inhibited the activity of connexin channels.In good'sph buffer (MES (4-morpholine ethane sulfonic acid), HEPES and taps (3 - {[2-hydroxy-1,1-In bis (hydroxymethyl) ethyl] amino] - 1), the pH dependent activity of the linker channel demonstrates this.Propane sulfonic acid), they have a common sulfamate moiety, and there is no sulfamate.Part (of the pH buffer does not have pH dependent channel activity.Therefore, taps is more used for cell culture Experiments on flagellate algae in the substrate.
Preparation method of taps solution (about 1-2l)
(1) Prepare 0.1M solution (a): taps 24.328g/deionized water 1000ml
(2) Prepare 0.1M NaOH solution (b): NaOH 4G / deionized water 1000ml
pH 4.6 |
1,000ml(A)+0ml(B) (A):(B)=5:0 |
pH 7.8 |
1,000ml(A)+200ml(B) (A):(B)=5:1 |
pH 8.3 |
1,000ml(A)+400ml(B) (A):(B)=5:2 |
pH 8.6 |
1,000ml(A)+600ml(B) (A):(B)=5:3 |
pH 9.0 |
1,000ml(A)+800ml(B) (A):(B)=5:4 |
* temperature 20 degrees.
* use a pH meter if you need to adjust to a specific pH.
* do not want to join Na, please use KOH.
Hubei new Desheng Material Technology Co., Ltd. is specialized in the production of biological buffer taps products, and has 15 years of production experience. We have a professional R & D team, which can solve the follow-up problems for you. We can also customize the indicators of the products, please contact us, welcome your consultation!
Contact Person: Miss. Ankiwang
Tel: 8615071057538
Fax: 86-0711-3704589