Common Problems for Sodium Heparin in the Application of Blood Collection Tubes
1. Heparin sodium blood tubes are usually used in blood test, what kind of test suitable for?
Clinically, heparin sodium is mainly used for the prevention and treatment of thrombosis or embolic diseases, disseminated intravascular coagulation caused by various reasons,and hemodialysis, extracorporeal circulation, catheterization, microvascular surgery, etc. In the same time, it’s also used as anti-coagulant in blood testing and instrument. So the tubes with heparin sodium are used for anti-coagulation of blood.
2. How to use heparin sodium in tubes when doing research?
Usually put the heparin sodium on pipe, and dry it in oven within 10-300s.The time is based on volume of heparin sodium.
3. What is the purpose of drying? Can it be used directly without drying?
The purpose of drying is mainly to reduce the effect of heparin sodium on concentration of drug in plasma, and it can be used directly without drying.
4. Is heparin sodium resistant to high temperature?
Yes, sodium heparin is generally resistant to high temperature, and can be dried in an oven at 80 to 100 °C. It’s better to use the dried sodium heparin within one week.
5. Why is it best to use serum as the specimen when using sodium heparin to measure the content of protein?
When add heparin sodium in blood, it blocks the generation of thromboplastin and prevents blood coagulation, resulting in the inability of fibrinogen to be hydrolyzed and remains in plasma. When blood coagulated,the fibrinogen hydrolyzed into fibrin monomers and consumed,but the serum is separated out along with blood cells after centrifuging. In that case, the total protein content in plasma contains all proteins including fibrinogen, while the total protein content in serum does not contain fibrinogen, which should theoretically be lower than the total protein content in plasma. Therefore, it is best to use serum as the specimen when using sodium heparin to measure the content of protein.For some emergency specimens, or some patients who need to be measured urgently for other reasons,and plasma samples are needed to measure total protein, the content of fibrinogen should be deducted (generally 3% to 5%) after the total protein is measured. Only in this way can it be consistent with the total protein content measured by serum samples, and truly reflect the actual total protein content in the patient's body.
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