Mentioned that war epidemic technology has to mention that the important means to block the spread of the epidemic is nucleic acid detection. The raw material of the detection reagent is the key to the role of nucleic acid detection, and this core raw material is the Virus Transport Media. Many people will feel frightened and helpless when it comes to new coronary pneumonia. It is extremely contagious, leading to isolation for several months in 2020. During the quarantine period, it was also reported that the inspector of the laboratory of Wuhan Jinyintan Hospital was infected with the new coronavirus without contacting the patient. Why is this? Is there any way to solve it?
Virus Transport Media (inactivated and non-inactivated)
The inspectors at the Jinyintan Laboratory never contacted the patients, but only performed tests, Four of them were still infected.How did they get infected? Experts speculate that one possibility is that when performing high-risk operations such as laboratory tests, the patient's blood samples are exposed to the air to form an aerosol, and the four inspectors are carried by the aerosol Infected by a virus. If this is the case, then the virus is very powerful. Without contact with the patient, a little blood is outside and it may become an aerosol. The transmission routes can therefore be divided into contact transmission, droplet transmission, and air transmission, which may account for the main transmission routes. In response to this problem, Desheng has developed a virus inactivation Virus Transport Media, which greatly reduces the possibility of infection during the detection process.
The principle of nucleic acid detection of New Coronavirus is to expose the RNA of the virus through cell lysate, and then use real-time fluorescent RT-PCR for detection. Because the new coronavirus is very infectious, in order to protect the detection personnel and reduce the risk of infection, the virus needs to be inactivated.
Virus inactivation is to make the viral protein no longer have physiological activity, and lose the ability of infection, disease and reproduction. The main method is to inactivate the water bath at high temperature, that is, put the sample in the water bath box and inactivate it at 56℃ for half an hour. In addition, some nucleic acid detection kits come with virus inactivation Virus Transport Media, which can "kill" the virus and protect the RNA during the sample collection stage. This Virus Transport Media is prepared based on nucleic acid extraction lysate.
Because it is based on the preparation of accounting lysate, the role of guanidine hydrochloride, EDTA, TRIS and other reagents in this Virus Transport Media is to cleave the virus to release nucleic acids. Guanidine hydrochloride not only rapidly destroys the cell membrane, but also denatures the protein, allowing the protein to denature and precipitate, allowing the nucleic acid to get rid of the protein. EDTA chelating agent can combine with metal ions such as Mg2+, Ca2+, Mn2+, Fe2+, etc., and reduce the influence of metal ions on nucleic acid quality.
On the one hand, the virus inactivation Virus Transport Media directly cleaves the virus to release nucleic acid, and eliminates nucleic acid degrading enzyme (RNase) to prevent the degradation of virus RNA. On the other hand, the protein of the virus can be denatured, lose its activity and "dead", no longer infectious, and improve the safety of the transportation and detection stage.
In addition to the above-mentioned virus-inactivated Virus Transport Medias, Desheng also has non-inactivated Virus Transport Medias. There are also differences in the types of Virus Transport Medias used according to various testing needs. Desheng has been working hard to make its own slight contribution to the prevention and control of the epidemic, hoping that the motherland can prosper safely.
Contact Person: Miss. Ankiwang