Dipotassium EDTA is an anticoagulant commonly used in blood analyzers. It has the characteristics of good anticoagulation effect and less influence on the morphology of blood cells. Blood analysis is a routine test item in clinical medicine. The vast majority of patients undergo this test. From the beginning of manual detection to fully automatic detection. All demands are increasing.
Ethylenediaminetetraacetic acid dipotassium (EDTA-2K) ethylenediaminetetraacetic acid (abbreviated as Dipotassium EDTA) is an amino polyacid.It is a general-purpose strong complexing agent for forming complexes with most metal ions. The past research on Dipotassium EDTA complexes and chelation has focused on three aspects.
1. What attracts more research forces is the most stable metal complex of Dipotassium EDTA,the chelate of transition elements and rare elements.
2. A batch of complexes with moderate complexing strength, such as the accumulation of alkaline metals. Since it is difficult for general reagents to achieve the complexation of alkali metals, the complex of Dipotassium EDTA alkaline metals has attracted people's attention in particular;
3.Through the sequence of Dipotassium EDTA itself and co-alkali metal salts, the research on the affinity of Dipotassium EDTA acid radical to proton is focused.
Dipotassium EDTA is an amino polycarboxylic acid and calcium chelator.It has a great affinity for calcium ion in blood, which can effectively chelate calcium ion or remove calcium reaction sites in blood samples.The drop of calcium ion will block and stop the endogenous or extrinsic coagulation process, thereby preventing the coagulation of blood samples.Each 0.8mg can anticoagulate 1ml of blood. Except that it cannot be used for the determination of calcium and sodium containing substances in plasma, it is suitable for various anticoagulation. Dipotassium EDTA can also complex some ions in plasma to make certain of proteins or nucleic acid substances more stable.But the interference of the formation of chromium compounds on samples and experiments should be considered.
Dipotassium EDTA is suitable for general hematological tests, but not for platelet count. Because it affects platelet aggregation and blood cell phagocytosis. It is not suitable for coagulation imaging and platelet function tests, nor for calcium, potassium, NaFe, alkaline phosphatase, creatine Kinase and leucine aminopeptidase assays and PCR assays.
Dipotassium ethylenediaminetetraacetate (EDTA-K) is additives of vacuum blood collection tubes,anticoagulation of 2ml blood requires 4mg of Desheng ethylenediaminetetraacetic acid dipotassium (EDTA-K). Because the concentration is low, in order not to dilute the blood, 20ul of an aqueous solution containing 200g/L of dipotassium ethylenediaminetetraacetate (EDTA-K) is generally used in each blood collection tube.
When the water is evaporated on the tube wall,the Dipotassium EDTA in the tube to crystallize quickly. The blood collection tubes must be turned upside down for at least 8 times, so that the crystallized dipotassium ethylenediaminetetraacetate (EDTA-K) can be fully dissolved and mixed in the blood.
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