Dipotassium EDTA as an additive for blood collection,edta blood tube

[product name] EDTA dihydrate

[package specification] plastic bottle, 500g / bottle [product performance] white crystal powder, odorless, soluble in water, easy to absorb moisture, molecular weight of 404.6, pH value of 4.8 ± 1 (5% aqueous solution, g / ml), solubility in water ≥ 60% (g / ml, 25 ℃). Stable at room temperature. The main content of EDTA in the company is ≥ 99.0%. See the product test report for other indicators.

[scope of application] EDTA salt can protect the cell composition of blood, not affect the white blood cell count and size, has the least impact on the morphology of red blood cells, and can inhibit the aggregation of platelets, which is suitable for general hematology test. In addition to the separation and test of platelets, it is not suitable for coagulation test and platelet kinetic energy test, nor for the determination of calcium ion, potassium ion, sodium ion, iron ion, alkaline phosphatase, creatine kinase and leucine aminopeptidase and PCR test. International Committee for hematology Standardization (ICSH) recommended EDTA as anticoagulant for blood cell count and classification. Compared with EDTA, it has lower solubility and slower anticoagulation speed.

[note] the key to the preparation of the whole blood test specimen is to fully and completely anticoagulant, so it is necessary to reverse it for 5 to 8 times as soon as possible after blood collection to prevent the formation of small blood clots. Especially when the ambient temperature of blood collection is higher than 25 ℃, the mixing of blood and EDTA must be timely and sufficient, otherwise it will easily lead to blood coagulation or local coagulation. The anticoagulation of EDTA salt is not irreversible, so the test should be completed within 24 hours after blood collection in EDTA anticoagulation test tube, otherwise the test result may be wrong.

The preservation time of EDTA salt anticoagulant samples was 48 hours at 4 ℃ and no more than 24 hours at 23 ℃. Platelet count can be preserved for 24 hours at 4 ℃. Enough EDTA is needed for anticoagulation, but excessive EDTA may change the morphology of blood cells, so it is very important to control the amount of EDTA. EDTA salt can inhibit platelet aggregation, but it can not completely prevent platelet aggregation.

In the blood routine test of a few patients, platelets are lower than normal value, which may be caused by false aggregation of platelets. In this case, sodium citrate anticoagulant can be used for platelet test. This situation has nothing to do with the quality of EDTA anticoagulant, but has much to do with the patient's blood characteristics and clinical examination.

EDTA dipotassium can be used in combination with sodium fluoride and separation gel. When EDTA dipotassium / sodium fluoride is used in combination, 1.2-2.0mg dipotassium and 2-4mg sodium fluoride can be added to each ml of blood.

When EDTA dipotassium was mixed with separating glue, the dosage of dipotassium remained unchanged, and the amount of separating glue added to each tube was 0.8-1.2g. The anticoagulant effect, silicification of the tube wall, centrifugation conditions and the quality of the separating glue will affect the blood separating effect when they are used together with the separating glue.

It is suggested that high quality plasma samples can be obtained by using the separation gel produced by our company. It is suggested to use γ - ray irradiation at a dose of 8-25kgy. The irradiation dose can be determined by the initial colony number.

[warning and prevention] when EDTA dipotassium has impurities, foreign matters, abnormal smell, color, serious moisture absorption and the expiry date is exceeded, it is forbidden to use it. Do not continue to use EDTA when it is turbid.

This product is a chemical reagent with strong corrosiveness. It is forbidden to touch it directly.

When splashing on the skin, wash with plenty of water. Splashing into eyes can be washed with plenty of water immediately. In case of emergency, it should be sent to hospital for examination and treatment immediately.

[storage conditions] the product can be sealed and stored in a cool and dry place, away from light and moisture. The aqueous solution of this product can be sealed and stored in sterile state at room temperature. It is recommended that the aqueous solution of this product be prepared and used immediately, and not stored in solution state for a long time.

[validity period] 3 years