Dipotassium EDTA is often used in combination with serum separation gel and sodium fluoride
Dipotassium EDTA is mainly used as an in vitro blood anticoagulant and is widely added to vacuum test tubes. It is often used together with serum separation gel and sodium fluoride to make the detection results more accurate and improve efficiency. However, it is not added to the same test tube at the same time, the effect is the same, and the items checked are different for different additives. Below, I will specifically introduce to you the functions of dipotassium EDTA, serum separation gel and sodium fluoride.
1. Dipotassium EDTA + serum separation gel
If only dipotassium EDTA is added to the test tube, it is an ordinary blood routine test tube, and its function is to prevent blood coagulation. But it is added to the test tube together with serum separation gel, which is generally used in nucleic acid detection tubes.
Nucleic acid detection tubes are used for the collection, transportation and storage of venous blood samples for nucleic acid detection, mainly for DNA amplification detection of HBV DNA, HCV, and HIV, and serum separation gel can well isolate hemoglobin in red blood cells to prevent Nucleic acid detection experiments are interfering.
2. Dipotassium EDTA + Sodium Fluoride
Can sodium fluoride effectively inhibit glycolysis and keep blood sugar concentration relatively stable, so it is considered an ideal blood sugar test specimen preservation agent, while dipotassium EDTA can prevent blood coagulation due to its own chelating properties , and the dissolution rate is fast. When used together with sodium fluoride in the preparation of anticoagulation tubes, it can be dried at 100 degrees, and the anticoagulation effect can still be maintained without worrying about being decomposed. The preparation conditions are also easier to control, which is convenient for production to improve efficiency.
Dipotassium EDTA is used together with serum separation gel and sodium fluoride. The amount of dipotassium EDTA should be noted. Generally, 4 mg of EDTA needs to be added to anticoagulate 2 ml of blood. For dipotassium acetate, usually 20 μl of an aqueous solution containing 200 g/L of dipotassium ethylenediaminetetraacetate is pre-placed in each blood collection tube. In addition, medical staff are required to turn upside down as soon as possible after blood collection, so that they are fully exposed to the additives, but they should not be shaken too violently, which may easily cause hemolysis.
Desheng Biochemical has always been a veteran manufacturer of blood collection tube additives, focusing on a series of products, such as serum separation gel in blood collection tubes, heparin series, EDTA series, to meet the different needs of customers. Today, all kinds of products have been exported to overseas, opening up a "road of raw materials for blood collection tubes" of its own. If you have needs in this regard, please visit our website !
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