Do you know how much excitation solution is needed for acridine ester?

Acridine ester is a compound widely used in biochemical and molecular biology experiments, commonly used for labeling proteins, antigens, antibodies, nucleic acids, etc. During the experiment, acridine ester needs to be used together with the excitation solution to achieve maximum effectiveness. So, what is the excitation solution required for acridine ester? This article will explore the required excitation solution based on the characteristics and applications of acridine esters.

1、 Characteristics of Acridine Esters

Acridine ester is an acridine ester containing a - NHS group, with a chemical formula of C30H26N2O9S and a molecular weight of 590.6. Its structure contains sulfonic acid groups, which increase its water solubility, making it water-soluble with markers formed by antibodies, nucleic acids, etc., and subsequent luminescence detection can be carried out in water. Acridine ester is stable in acidic solution (pH 4.8) and can be stored at room temperature for 4 weeks with protein conjugates. Its photoquantum yield does not decrease, and the freeze-dried product can be stored for more than one year at -20 ° C.

2、 Application of Acridine Ester

Acridine esters are widely used as chemiluminescent agents in direct chemiluminescence immunoassay, for the detection and analysis of antigens, antibodies, proteins, etc. Its use can greatly improve the sensitivity and specificity of detection, providing a powerful tool for biological research.

3、 Exciting solution required for acridine ester

Acridine ester needs to be used together with the excitation solution in the experiment to achieve maximum effect. So, what is the excitation solution required for acridine ester? In fact, there is no fixed formula for the excitation solution required for acridine ester, but it needs to be selected based on specific experimental requirements and conditions. Here are some commonly used excitation solution formulas:

0.1M phosphate buffer solution (PBS): PBS is a commonly used buffer solution that can stabilize pH and prevent protein denaturation. In the experiment of acridine ester, PBS can be used as the basic excitation solution to adjust the pH value and ion strength of the sample.

0.1M sodium hydroxide solution: Sodium hydroxide solution is a strong alkaline solution that can be used to adjust the pH value of the sample. In the experiment of acridine ester, sodium hydroxide solution can be used to adjust the pH value of the sample to alkaline, thereby promoting the luminescence reaction of acridine ester.

0.1M hydrochloric acid solution: Hydrochloric acid solution is a strong acidic solution that can be used to adjust the pH value of the sample. In the experiment of acridine ester, hydrochloric acid solution can be used to adjust the pH value of the sample to acidity, thereby promoting the luminescence reaction of acridine ester.

1% sodium dodecyl sulfate (SDS) solution: SDS is a surfactant that can be used to improve the stability and solubility of samples. In the experiment of acridine ester, SDS solution can be used to dissolve the sample, thereby improving the luminescence efficiency of acridine ester.

The above are some commonly used excitation solution formulas, and in practical applications, it is necessary to choose the appropriate excitation solution according to experimental requirements and conditions. At the same time, attention should also be paid to controlling the pH value and ion strength of the excitation solution to ensure the luminescence effect of acridine ester.

4、 Summary

Acridine ester is a compound widely used in biochemical and molecular biology experiments, commonly used for labeling proteins, antigens, antibodies, nucleic acids, etc. During the experiment, acridine ester needs to be used together with the excitation solution to achieve maximum effectiveness. The excitation solution required for acridine ester does not have a fixed formula, but needs to be selected based on specific experimental requirements and conditions. The commonly used excitation solution formulas include PBS, sodium hydroxide solution, hydrochloric acid solution, and SDS solution. At the same time, attention should also be paid to controlling the pH value and ion strength of the excitation solution to ensure the luminescence effect of acridine ester.

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