Exploration of the advantages of biological buffer BICINE

In the fields of biochemistry and molecular biology, buffering agents are crucial for maintaining the stability of solution pH, as they are related to the activity and functional performance of biomolecules. BICINE buffer, As a commonly used biological buffer, it has demonstrated significant advantages in numerous application scenarios due to its unique properties.

Excellent buffering performance

(1) Suitable and broad pH buffering range

The pH buffer range of BICINE is between 7.6-9.0, which is close to the weak alkaline range in the physiological environment of organisms and meets the pH requirements of most biochemical reactions and biological processes. Taking enzymatic reactions as an example, most enzymes have the highest activity at a specific pH value. Beyond the appropriate range, enzyme activity will be inhibited or even inactivated. The BICINE buffer system can provide a stable weakly alkaline environment, ensuring the smooth progress of numerous enzyme catalyzed biochemical reactions. For example, in serum guanine enzyme assays, it accurately maintains the pH conditions required for the reaction, ensures enzyme activity stability, and makes detection results accurate and reliable.

(2) Powerful buffering capacity and stability

Faced with the addition of external acidic and alkaline substances or changes in acidity and alkalinity during the reaction process, BICINE, with its structural characteristics, can quickly respond and neutralize fluctuations in acidity and alkalinity, maintaining a relatively constant pH value of the solution. In protein electrophoresis experiments, as electrophoresis proceeds, an electrolytic reaction occurs at both ends of the electrode, causing a change in the pH value of the solution. BICINE buffer can effectively counteract this change, ensuring that protein molecules migrate in a stable pH environment, thereby obtaining clear and accurate electrophoresis patterns, greatly improving the reliability and reproducibility of experimental results.

Biocompatible

(1) Low interference to biomolecules

BICINE has relatively inert chemical properties and is not easily reacted with key molecules in biological systems, such as proteins, nucleic acids, enzymes, etc., under common biochemical experimental conditions. It does not interfere with the normal structure and function of biological molecules. In protein crystallization experiments, the use of BICINE buffer can provide a stable pH microenvironment for protein molecules without affecting the interactions and ordered arrangement between protein molecules, which helps to obtain high-quality and high-resolution protein crystals and lays the foundation for subsequent protein structure analysis and functional research.

(2) Reliable applications in cell culture

In the field of cell culture, cell growth and metabolism are extremely sensitive to the pH value of the culture environment. BICINE can be added to cell culture media to effectively maintain stable pH values and create a suitable microenvironment for cell survival and proliferation. It can not only inhibit the accidental growth of mycoplasma in animal tissue culture, but also prevent the precipitation of iron salts in bacterial culture medium, ensure the purity and stability of the cell culture process, promote healthy cell growth, and improve the success rate and stability of cell culture experiments.

Excellent physical and chemical properties

(1) High solubility

BICINE is a white crystalline powder that is easily soluble in water and common polar organic solvents such as DMF, DMSO, etc. This characteristic greatly facilitates the preparation process of buffer solutions. Experimenters can easily dissolve BICINE in the corresponding solvent according to different experimental needs, and quickly prepare a buffer solution with the required concentration and pH value. The operation is simple, efficient, and reduces experimental preparation time and errors.

(2) Strong chemical stability

The special chemical bonds and functional groups in the BICINE structure endow it with good chemical stability, which can effectively resist chemical and enzymatic hydrolysis under conventional experimental conditions. In nucleic acid operation experiments, such as gel electrophoresis and enzymatic reaction, BICINE buffer solution will not decompose or deteriorate due to the influence of reaction conditions, and always maintains a stable buffer capacity to ensure the integrity and activity of nucleic acid molecules are not disturbed during the experiment, and ensure the smooth progress of the experiment and accurate results.

Unique chelating properties

The specific functional groups in the BICINE molecular structure endow it with the ability to chelate metal ions to a certain extent. In the field of cosmetics, the ingredients are complex and may contain metal ions such as iron and copper, which can affect product stability and efficacy, and even irritate the skin. BICINE can chelate with these metal ions to form stable complexes, reducing the adverse effects of metal ions on cosmetics, such as preventing metal ion catalyzed oxidation reactions and extending product shelf life; Reduce the irritation of metal ions to the skin and enhance the safety and mildness of the product. In terms of soil remediation, BICINE can specifically chelate heavy metal ions such as copper, cadmium, lead, etc. in the soil. While removing heavy metal pollution, it does not react with essential nutrients such as calcium and magnesium for plant growth in the soil, maintaining soil fertility and achieving efficient and green soil remediation, providing strong support for environmental protection.

The chloride ion content of bicine buffer produced by Hubei Xindesheng Material Technology Co., Ltd. is less than 0.1%, and all indicators meet relevant standards. In addition to bicine buffer, Desheng actively researches and develops dozens of biological buffer such as TRIS and hepes commonly used in the market. If you are interested, please click on the Desheng official website to learn more details!