Product Details
Place of Origin: hubei
Brand Name: desheng
Certification: ISO9001
Model Number: CAS 150-25-4
Payment & Shipping Terms
Minimum Order Quantity: 20kg
Price: Detailed discussion
Packaging Details: 20kg / box
Delivery Time: 5 days
Payment Terms: L/C, D/P, T/T, Western Union
Supply Ability: 5t/week
Appearance: |
White Powder |
Model: |
Bicine |
Manufacturer: |
Desheng |
Packing: |
500g / Box |
Appearance: |
White Powder |
Model: |
Bicine |
Manufacturer: |
Desheng |
Packing: |
500g / Box |
Bicine buffer is a widely used biological buffer commonly used in important scientific research sites such as laboratories. It is also known as dihydroxyethylglycine and has a CAS number of 150-25-4. It has superior buffering performance like trimethylaminomethane and belongs to amino acid derivatives. The aqueous solution is weakly acidic, with a pH buffering range of 7.6-9.0. It is soluble in water and commonly used organic solutions such as DMF, DMSO, DMAc, etc.
| Product Name | Bicine buffer solution |
| Appearance | White crystalline powder |
| Product function | Maintaining pH stability, providing an ionic environment, preventing protein denaturation, etc |
| Approaches to application | Biological laboratories, chemical laboratories, medical laboratories, etc |
| Product advantages | Purity ≥ 99%, good water solubility, stable production process |
| Storage condition | Avoid moisture, seal and store in a dry place away from light |
| Manufacturer | Hubei Xindesheng |
The role of Bicine buffer solution
1. Maintaining pH stability: Bicine buffer is a weakly alkaline buffer that can effectively maintain the pH stability of a solution. During the experiment, the pH value of the solution may change due to the addition of reactants and products. The presence of Bicine buffer can effectively stabilize the pH value of the solution and ensure the accuracy of experimental results.
2. Provide ion environment: Bicine buffer is an ion buffer that can provide a suitable ion environment. In some experiments, a specific ion environment is required to conduct. And Bicine buffer can provide a suitable ion environment to ensure the smooth progress of the experiment.
3. Preventing protein denaturation: In biological laboratories, proteins are important research objects. However, proteins may undergo denaturation under certain conditions, leading to inaccurate experimental results. Bicine buffer can effectively prevent protein denaturation and ensure the accuracy of experimental results.
bicine buffer
The application of Bicine buffer in the laboratory
1. Biological laboratory
Bicine buffer is widely used in various experiments in biological laboratories. For example, in protein electrophoresis experiments, Bicine buffer can be used as the electrophoresis solution to provide a suitable ion environment and ensure the smooth progress of the electrophoresis experiment. In addition, in protein purification experiments, Bicine buffer is an effective eluent commonly used for protein purification and separation.
2. Chemical laboratory
In chemical reactions, Bicine buffer can be used as a reaction medium to provide a suitable ionic environment and ensure the smooth progress of the chemical reaction. In addition, in analytical chemistry experiments, Bicine buffer can also be used as a standard solution to help accurately measure experimental results.
3. Medical laboratory
In immunoassay experiments, Bicine buffer can be used as a washing solution, and it is often used in the separation and purification of antigens or antibodies. In addition, Bicine buffer can also be used as a culture medium in cell culture experiments, providing a suitable ionic environment to ensure normal cell growth and reproduction.
Product packaging
Bicine buffer has a wide range of applications, as it can effectively maintain the pH stability of the solution, provide a suitable ionic environment, prevent protein denaturation, etc. It is also important to purchase high-quality bicine buffer. Hubei Xindesheng Material Technology Co., Ltd. is a professional manufacturer of bio buffering agents such as bicine. Our professional R&D personnel ensure high product purity, low impurities, good water solubility, and strong buffering ability. If you need it, please feel free to contact us!
FAQ
What is the function of the bicine buffer?
It is a zwitterionic, bis(2-hydroxyethyl)amine buffer that is useful for a pH range of 7.6 – 9.0. It is commonly used in protein crystallization, in the study of enzymatic reactions, and for electrophoresis.
How do you make a Bicine buffer?
Bicine (1 M, pH 8.26) Preparation and Recipe
Prepare 800 mL of dH2O in a suitable container.
Add 163.17 g of Bicine to the solution.
Adjust solution to desired pH with 10N NaOH.
Add dH2O until the volume is 1 L.
What is the bicine buffer pH 8.5?
Bicine Buffer 1M, pH 8.5 is a general purpose Good's Buffer that has seen use in tissue culture, phosphorylation and photophosphorylation, fixative transmission electron microscopy, and protein synthesis. This buffer can prevent binding to non-receptor materials.
What is the concentration of bicine buffer?
Description. Bicine buffer has a concentration of 0.3M and a pH at 8.5. Bicine is a zwitterionic amino acid active buffer. It is recommended for work in low temperatures of biochemical analysis and is used in molecular biology work.
