HEPES solution is a weak acid, the Chinese name is hydroxyethyl piperazine ethylsulfonic acid, is an amphoteric buffer in organic chemical industry. However, compared with other buffers, the decomposition constant of HEPES does not change much with temperature, which makes HEPES buffer a buffer that can maintain the structure and function of enzyme at low temperature.
HEPES buffer can control the constant pH range for a long time and has no toxic effect on cells. It is often used in cell culture. In order to improve the culture environment of BHK-21 cells, maintain the stability of 146S antigen of foot-and-mouth disease virus, and increase the content of complete virus particles in vaccine, some experts studied the buffer system of culture medium. In the study, the stability of HEPES on virus antigen was compared to evaluate its protective effect.
Package of Desheng HEPES biological buffer in big barrel
According to the experimental results, the virus titer of HEPES was higher than that of HEPES without biological buffer. At 37 ℃, TCID50 of virus without biological buffer changed more than that of virus with biological buffer. However, it should be noted that when HEPES is exposed to light, hydrogen peroxide will be produced, which is toxic to cultured cells or other bioactive substances. Therefore, HEPES should be used as a buffer as far as possible In dark condition.
Therefore, biological buffer can effectively improve the buffer capacity of virus culture medium, provide a suitable environment for virus and promote the stability of virus particles. If you need to buy HEPES buffer solution, please find Desheng technology, a biochemical reagent company integrating scientific research, production and sales. Direct sales from manufacturers can save you a lot of purchase costs.
