How to use heparin sodium as anticoagulant
As a natural anticoagulant in mammals, heparin has a wide range of applications, whether in clinical medicine or cosmetics, or in drug preparation. As a natural ideal anticoagulant, it has naturally attracted the attention of all countries in the world and has gradually become one of the main raw materials exported by China. So, since heparin sodium is so popular, how is it used as an anticoagulant? Let's give you a detailed introduction.
When it comes to how to use it as an anticoagulant, it is actually inseparable from its extraction method and production process. At present, the main source of heparin sodium is from pig intestines, with high content. The domestic pig industry is developed and can supply heparin to the maximum extent. Then the extracted heparin is purified to prepare the required heparin sodium.
As an anticoagulant, the preparation process of heparin sodium has the following steps:
1. Raw material treatment: clean the extracted fresh pig intestines, scrape the mucous membrane, and then put them into a blender for mixing and standby.
2. Heating enzymolysis: at this time, put the stirred intestinal mucosa into a heating vessel and add water to mix and stir, then add trypsin and weak alkaline solution in turn, fully adjust the ph value until the solution ph reaches 8-10, and then continue heating, and when heating to a certain time and temperature, filter to obtain the filtrate.
3. Cooling and adsorption: cool the filtrate obtained, remove the floating oil on the upper layer after cooling, and then add resin to stir for adsorption for about 6 hours. After adsorption, filter out the resin.
4. Elution: put the collected resin into the elutor for washing, add a certain proportion of sodium chloride solution, keep the temperature at about 50 ℃, and mix for 3 hours before collecting the eluent.
5. Sedimentation: filter the obtained eluate, then put it into the appliance, add alcohol and stir to adjust the ph value evenly, the range should be kept at 7-8, leave it for about 10 hours, seal and precipitate, then filter the precipitate, and use it for standby.
6. Dehydration and drying: put the sediment into a drying oven to dry, and then add heparin sodium into the test tube as an anticoagulant, and then add it as a solution.
The above is the introduction of how heparin sodium can be used as an anticoagulant. Its manufacturing process is the key to determine the quality of anticoagulant, so special attention should be paid to the details in the production process. Desheng, as a manufacturer of heparin sodium in China, has stable production process and simple method. It will not affect the process of the experiment when used as an anticoagulant in vitro. If you have any intention, please click the website for consultation!
