Keywords: biological buffer, CAPS, 3-cyclohexylaminopropanesulfonic acid, Desheng
CAPS, the full name of 3-cyclohexylaminopropanesulfonic acid, Good Buffer Solutions CAPS. It is widely used and can be divided into two categories. CAPS needs better purity when it is used as a biological buffer. Generally, it is necessary to analyze the purity before use. When it is used in industry, the purity requirement is lower, but different applications require different treatments.
3-Cyclohexylaminopropanesulfonic acid (CAPS) is mainly used in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits, as a buffer for enzyme chemistry and HPLC separation of basic drugs. 3-Cyclohexylaminopropanesulfonic acid (CAPS) can also support alkaline phosphatase activity and inhibit the growth of Aeromonas at a pH of 10.5, and dissolve in deionized water and adjust the pH to 11.0 for Purification of fibronectin.
In the field of capillary electrophoresis analysis, 3-cyclohexylaminopropanesulfonic acid (CAPS) is used to prepare running buffer (background electrolyte solution) in capillary electrophoresis, as a terminal electrolyte, it can realize 2-nitrobenzoic acid and 3-nitro Online electric enrichment and separation of benzoic acid.
In nucleic acid extraction,3-cyclohexylaminopropanesulfonic acid(CAPS)and 3-[(3-cholamidopropyl)dimethylammonium]-1-propanesulfonate(CHAPS), 3-(cyclo Hexylamino)-2-hydroxy-1-propanesulfonate (CAPSO), and 2-(cyclohexylamino)ethanesulfonate (CHES), etc. are used to prepare a solution for nucleic acid hybridization, which can reduce the non-specific hybridization products. The yield increases the specificity of nucleic acid hybridization while maintaining the yield of the target hybridization product. It has important value in the identification of specific pathogens, diagnosis of genetic diseases, and analysis of gene sequences.
4-Cyclohexylaminopropanesulfonic acid (CAPS) is also an important water-soluble modifier. It can be reacted with polyisocyanate under very mild reaction conditions and in the presence of a suitable neutralizing amine to prepare it for use in water Thus, the prepared polyisocyanate product can be emulsified in water into a finely dispersed form and storage stable.
In addition to the above uses, CAPS is also used to improve water-based coatings, flux for manufacturing welding materials, and air conditioning equipment.Heat exchange carrier, metal lithium manufacturing process raw materials, pyrotechnics, dry batteries, etc. Desheng is a manufacturer specializing in the production of reagents. Its experience in R&D and production of blood collection tube additives, biological buffers, in vitro diagnostic reagents, and chemiluminescent reagents is quite mature, and it provides customers with high-quality reagent raw materials.
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