Hubei Xinde Sheng has added two new enzymatic substrates: EPS-G7 and DGGR

In the field of core raw materials for in vitro diagnostic reagents, the quality of enzymatic substrates directly impacts the accuracy and reliability of detection methods. Hubei Xinde Sheng Materials Technology Co., Ltd. has recently introduced two new products: EPS-G7 amylase substrate and DGGR lipase substrate. These substrates are specifically used for the activity detection of α-amylase and lipase, respectively, serving as key components in diagnostic kits for pancreatic diseases.

EPS-G7: The substrate for amylase measurement recommended by IFCC

The chemical name of EPS-G7 is 4,6-ethenyl-p-nitrophenyl-α-D-maltose heptaglycoside, appearing as a white to light yellow powder with good water solubility. The product can achieve a purity of over 95%, requiring storage under light-protected and dry conditions at -20°C, with a shelf life of 2 years.

EPS-G7 is a dedicated substrate for α-amylase assays recommended by the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC). The assay principle involves: α-amylase hydrolyzing EPS-G7 to produce p-nitrophenyl oligosaccharides, which are further hydrolyzed by α-glucosidase into p-nitrophenol. By measuring the rate of absorbance change at 400 nm, the amylase activity can be calculated.

Compared to traditional PNP-G7 substrates, the 4,6-ethylene modification in EPS-G7 blocks the non-reducing end, effectively preventing direct hydrolysis of the substrate by α-glucosidase and enhancing substrate stability. This structural feature ensures more reliable detection results.

DGGR: Lipase highly specific chromogenic substrate

The chemical name of DGGR is 1,2-di-O-moleyl-rac-glycerin-3- (6-methylisoquinoline glutarate), which appears as a red to dark red powder or solid and is soluble in organic solvents such as DMSO and ethanol. The CAS number is 195833-46-6, with a purity of not less than 95%. It also needs to be stored under dark and dry conditions at minus 20 degrees Celsius, with a shelf life of 2 years.

DGGR is a highly specific chromogenic substrate for lipase, and its molecular structure has been carefully designed to be specifically recognized and hydrolyzed by lipase, releasing chromophores. Lipase activity in serum can be quantitatively detected by colorimetric method. The advantage of this substrate is its high selectivity towards lipase, minimal interference from other esterase substances, and the detection results can truly reflect the lipase activity level in the sample.

Clinical application value

The two substrates have clear application directions in clinical diagnosis. EPS-G7 is mainly used for quantitative detection of alpha amylase activity in human serum, plasma, or urine in vitro. It has important clinical significance in the initial screening of acute pancreatitis, functional evaluation of chronic pancreatitis, salivary gland suppuration or duct blockage, and auxiliary diagnosis of mumps. The test results can provide key basis for differential diagnosis of acute abdomen and comprehensive evaluation of pancreatic function.

The substrate of DGGR lipase forms a good complementary project with EPS-G7. Alpha amylase and lipase are routine combined detection indicators for pancreatic diseases, and their activities are significantly elevated during acute pancreatitis attacks. The specificity of detecting amylase alone is limited, and the elevation of certain non pancreatic amylase sources may interfere with the judgment. However, lipase has stronger tissue specificity, and combined detection can effectively improve the sensitivity and specificity of diagnosis. The matching supply of two substrates provides convenience for reagent kit manufacturers to purchase uniformly.

Quality assurance and supply capability

Hubei Xindesheng Material Technology Co., Ltd. already has multiple mature product lines in the field of in vitro diagnostics, including chromogenic substrates, chemiluminescence reagents, enzyme preparations, etc. The addition of EPS-G7 and DGGR products further expands the coverage of enzyme detection related raw materials. The product follows standard specifications in terms of purity control and batch stability, and can meet the quality requirements for core raw materials in the production of in vitro diagnostic kits. If you have any purchasing needs in the near future, please feel free to contact me at any time!