Introduction to the Performance, Formulation, and Addition of
Dipotassium Ethylenediaminetetraacetic Acid
Dipotassium ethylenediaminetetraacetate, as a commonly used reagent in clinical blood testing tubes, is suitable for various biochemical tests and provides effective value reference for clinical diagnosis, analysis of data, and treatment due to its ability to form stable complexes with calcium substances in blood samples, which can interfere with the coagulation process and prevent blood coagulation. Let's have a detailed discussion here.
1、 Performance of potassium ethylenediaminetetraacetic acid
Its appearance is white crystal powder, odorless, and soluble in water. As the finished product is in powder form, it is easy to absorb moisture, with a main content of over 99%. It is of analytical purity and can be prepared into a solution to be sprayed on the inner wall of the test tube when used. As it does not affect cellular components and can fully inhibit platelet aggregation, it is widely recognized in clinical use as a blood treatment reagent and is also recommended as an ideal anticoagulant reagent in the industry.
EDTA potassium powder
2、 Preparation of potassium ethylenediaminetetraacetic acid solution
Based on its performance, it can be determined that it is easily soluble in water, so the preparation process is also very simple. Prepare deionized water to dissolve to the required capacity. Due to the possibility of powder residue on the bottle mouth or inner wall of the instrument during the preparation process, in order to avoid excessive concentration or dilution of deionized water, the amount of potassium dioxide solution added to the test tube will be controlled. If it is necessary to increase the amount of potassium dioxide added, the preparation concentration can be increased first before considering whether to increase the spraying frequency. During this process, attention should be paid to formulating according to the test tube standard to avoid excessive concentration that may cause the experiment to fail.
3、 Addition amount of potassium ethylenediaminetetraacetic acid
According to industry standards, the addition amount is 1.5 to 2.2mg of potassium ethylenediaminetetraacetate per milliliter of blood. When preparing the reagent, the median value is 1.8mg/ml. Due to different experimental requirements, the addition amount can also be flexibly adjusted during use. Additionally, it should be noted that potassium ethylenediaminetetraacetic acid can be used in combination with sodium fluoride. When they are placed together in a test tube, the amount added may change, usually by adding 1.2-2.0 milligrams of potassium dioxide per milliliter of blood, and 2-
4 milligrams of sodium fluoride.
As the raw material supplier of blood collection tube additives, Desheng produces serum separation gel, EDTA potassium salt anticoagulant, accelerator, silicating agent and other products with stable performance and analytical purity. Adding them into the vacuum blood collection tube can effectively help experimental detection. If you are interested, please click on the website to inquire and purchase!
Contact Person: Miss. Ankiwang