α-glucosidase (EC.18.104.22.168), that is, α-D-glucoside glucose hydrolase, also known as glucosyltransferase GTase, its uses are very wide, including food and fermentation industry, chemical industry and medical applications, is a very promising enzyme preparation.
Glucosidase is widely present in animals, plants, bacteria and fungi, as long as it has carbohydrates as an energy source and has organisms with cell structures. There are two types of hydrolytic enzymes because the glucosidic bonds are classified into α-type and β-type. Among them, the hydrolysis of α-glucosidase can cut the α-1,4 glycosidic bond from the non-reducing ends of α-glucoside, oligosaccharides and dextran to release glucose; Glucose residues are transferred to another glucose or maltose substrate with α-1,6 glycosidic bonds, thereby obtaining non-fermentable isomaltose IMO.
The physiological significance of enzymes
α-glucosidase can hydrolyze oligosaccharides such as maltose and sucrose in the small intestine into glucose, and participate in regulating glucose metabolism and fat production in the body. In animal cells, α-glucosidase catalyzes the hydrolysis of glycogen to glucose in the lysosome, and participates in the metabolism of glycogen (glycogen is a branched polysaccharide formed by the combination of glucose and is a glucose in animal cells Main storage situation, glycogen is hydrolyzed first when hungry, followed by fat).
Application of enzyme preparation
1. Because of its key role in the metabolism of sugar in animal cells, recombinant human acid α-glucosidase is usually used to treat Pompe disease.
2. Used in the synthesis of functional oligosaccharides, using the transglycoside activity of α-glucosidase to synthesize energy oligoglucans, oligomalto-oligosaccharides, oligomeric isomaltose, oligo-cellulose oligosaccharides, etc. Functional sugars as prebiotics.
3. α-glucosidase can be used to screen active natural drugs.
It can be seen that α-glucosidase is a very important enzyme involved in sugar metabolism in organisms, and its enzyme activity is also an important indicator for body detection and disease diagnosis. To this end, Desheng Technology is also constantly exploring and innovating in the application of enzyme preparations.
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