Product Details
Place of Origin: China
Brand Name: DS
Certification: ISO9001
Model Number: AGH-1
Payment & Shipping Terms
Minimum Order Quantity: 50g
Price: TBD
Packaging Details: Plastic bottle
Delivery Time: 1-3 days
Payment Terms: L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability: 1000kg/month
Name: |
α-D-GLCOSIDE GLUCOHYDROLASE |
Color: |
White To Yellowish Amorphous Powder |
Storage: |
Store It Below -20℃ |
Michaelis Constant: |
6.3 X 10–4 M |
Most Appropriate PH: |
7.5 |
Cas No.: |
9001-42-7 |
Name: |
α-D-GLCOSIDE GLUCOHYDROLASE |
Color: |
White To Yellowish Amorphous Powder |
Storage: |
Store It Below -20℃ |
Michaelis Constant: |
6.3 X 10–4 M |
Most Appropriate PH: |
7.5 |
Cas No.: |
9001-42-7 |
1. Product specifications:
Product Name: | α-D-GLCOSIDE GLUCOHYDROLASE(AGH-1) |
Cas No. | 9001-42-7 |
Batch no: | E006-1 |
Appearance: | White to yellowish amorphous powder |
Specific Activity: | ≥10U/mg |
Stability: | Store it below -20℃, it could be stable for one year at least |
Molecular weight: | Around 65KD |
Isoelectronic point: | 5.2 |
Michaelis constant | 6.3 x 10–4 M(PNPG |
Inhibitor: | Not prohibited by Sodium Azide |
pH Stability: | 5.5~9.5(25℃,20hr.) (Fig.3-1) |
Most appropriate pH: | 7.5 (Fig.3-2) |
Most appropriate Temperature: | Above 65℃ (Fig.3-3) |
Heat stability: | Be stable below 55℃(15mins) (Fig.3-4) |
Application: is an important member of glucose metabolism pathway in organisms. Beta-glucosidase can participate in the metabolism of cellulose and many physiological and biochemical pathways, and alpha-glucosidase is directly involved in the metabolism of starch and glycogen. Abnormal functions of these enzymes can lead to metabolic diseases. At the same time, these enzymes are also targets of many drugs and inhibitors to regulate Glycochemistry in the human body.
Enzyme preparations
2.Enzyme activity testing method
Principle:
α-glycosidase p-Nitropheny1-a-D-glucopyranoside(PNPG)+H2O a-D-glucose+P-Nitrophenol (PNP)
PNP has specific absorption at 405 nm. The activity of PNP was calculated by measuring the change rate of absorbance at 405 nm.
Enzyme Activity Definition
An enzyme activity unit is the amount of enzyme that produces 1 micromole of PNP in one minute under the following reaction conditions, expressed as one unit.
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