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AGH-1 Enzyme Substrate Product White To Yellowish Amorphous Powder CAS 9001-42-7

Product Details

Place of Origin: China

Brand Name: DS

Certification: ISO9001

Model Number: AGH-1

Payment & Shipping Terms

Minimum Order Quantity: 50g

Price: TBD

Packaging Details: Plastic bottle

Delivery Time: 1-3 days

Payment Terms: L/C, D/A, D/P, T/T, Western Union, MoneyGram

Supply Ability: 1000kg/month

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Specifications
Highlight:

enzyme reactant complex

,

citric acid crystals

,

9001-42-7

Name:
α-D-GLCOSIDE GLUCOHYDROLASE
Color:
White To Yellowish Amorphous Powder
Storage:
Store It Below -20℃
Michaelis Constant:
6.3 X 10–4 M
Most Appropriate PH:
7.5
Cas No.:
9001-42-7
Name:
α-D-GLCOSIDE GLUCOHYDROLASE
Color:
White To Yellowish Amorphous Powder
Storage:
Store It Below -20℃
Michaelis Constant:
6.3 X 10–4 M
Most Appropriate PH:
7.5
Cas No.:
9001-42-7
Description
AGH-1 Enzyme Substrate Product White To Yellowish Amorphous Powder CAS 9001-42-7

 

 

1. Product specifications:

 

Product Name: α-D-GLCOSIDE GLUCOHYDROLASE(AGH-1)
Cas No. 9001-42-7
Batch no: E006-1
Appearance: White to yellowish amorphous powder
Specific Activity: ≥10U/mg
Stability: Store it below -20℃, it could be stable for one year at least
Molecular weight: Around 65KD
Isoelectronic point: 5.2
Michaelis constant 6.3 x 10–4 M(PNPG
Inhibitor: Not prohibited by Sodium Azide
pH Stability: 5.5~9.5(25℃,20hr.) (Fig.3-1)
Most appropriate pH: 7.5 (Fig.3-2)
Most appropriate Temperature: Above 65℃ (Fig.3-3)
Heat stability: Be stable below 55℃(15mins) (Fig.3-4)

 

Application: is an important member of glucose metabolism pathway in organisms. Beta-glucosidase can participate in the metabolism of cellulose and many physiological and biochemical pathways, and alpha-glucosidase is directly involved in the metabolism of starch and glycogen. Abnormal functions of these enzymes can lead to metabolic diseases. At the same time, these enzymes are also targets of many drugs and inhibitors to regulate Glycochemistry in the human body.

 

AGH-1 Enzyme Substrate Product White To Yellowish Amorphous Powder CAS 9001-42-7 0

Enzyme preparations

 

2.Enzyme activity testing method

 

Principle:

 

α-glycosidase p-Nitropheny1-a-D-glucopyranoside(PNPG)+H2O a-D-glucose+P-Nitrophenol (PNP)

PNP has specific absorption at 405 nm. The activity of PNP was calculated by measuring the change rate of absorbance at 405 nm.

 

Enzyme Activity Definition

 

An enzyme activity unit is the amount of enzyme that produces 1 micromole of PNP in one minute under the following reaction conditions, expressed as one unit.

 

 

 

 

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