In biochemical testing, the detection of blood glucose, blood lipids, liver and kidney function, etc. usually adopts enzymatic photometry. Many detection principles are based on Trinder reaction. The required chromogen substrates and enzymes have requirements for the experiment, and the stability is not ideal. Polymer stabilizers to improve.
The chromogen substrates used in the Trinder reaction, such as TOOS, MAOS, ADPS, etc., belong to the sodium salt of aniline sulfonate. After being formulated into an aqueous solution, it cannot be exposed to the air for a long time and will be slowly oxidized. Therefore, it is usually stored as a powder reagent. Temporarily prepare a solution. On the other hand, enzymes have higher requirements on the temperature and pH of the reaction system, and they are prone to inactivation even when the concentration is too low. So improving their stability is very valuable.
Chromogen substrate MAOS powder
Through research, it is found that a polymer with a special structure can significantly improve the stability of chromogen substrates and enzymes. The polymer has the general structural formula of R1 R2 R3, R1 is selected from olefin unsaturated groups or olefin unsaturated monomers; R2 is selected from ester group COO or ether group O; R3 is selected from polyethylene glycol, polyoxypropylene, Polypropylene glycol, polyacryl alcohol and other polymer groups.
The application of this polymer can increase its stability by interacting with enzymes and chromogen substrates, so that a variety of in vitro testing items can maintain the stability of enzymes and chromogen substrates under extreme environments (such as high temperatures). The detection result has less deviation and higher accuracy. It can be combined with other components to form a stable enzyme and chromogen substrate composition. It can be used in enzyme-catalyzed color reaction and in the preparation of in vitro detection products based on the principle of enzyme-catalyzed color reaction. And the preparation of enzymes and chromogen substrate stabilizers.
Applicable enzyme preparations include: peroxidase such as POD or HRP, cholesterol esterase, cholesterol oxidase, alkaline phosphatase, creatine kinase, glycerol kinase, phosphoglycerol oxidase, lipoprotein lipase, urate oxidase, acetone One or two or more of acid oxidase, glucose oxidase, creatinine hydrolase, and creatine hydrolase.
Trinder's chromogen substrates suitable for polymer stabilizers include: TOOS, TOPS, MAOS, MADB, etc. and their combinations 4-AAP, MBTH. The above-mentioned chromogen substrates and enzymes are commonly used reagents in enzymatic photometry. Desheng is a manufacturer and can provide a variety of chromogen substrates and enzyme preparations.
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