Company News About Purple EDTA tube can be used for blood sample collection in paternity testing
As an important application of modern biological science, paternity testing provides a scientific basis for resolving disputes over blood relationships. This process relies on DNA analysis, and sample collection is an important link in the entire identification chain. This article will focus on the commonly used sample type in paternity testing - blood, and how to use purple EDTA tubes for blood sample collection.
1、 Principles of paternity testing
The core of paternity testing is to compare the DNA sequences of individuals to determine their biological relationships. Everyone's DNA sequence is unique, except for genetic information from parents. Therefore, by comparing the DNA of suspected fathers, mothers, and children, scientists can calculate the degree of genetic information matching between the two and determine whether there is a parent-child relationship.
2、 The role of purple EDTA tube
Among various sample collection methods, blood is the preferred choice due to its high DNA content and ease of collection. However, blood collection naturally coagulates, which poses obstacles to DNA extraction. To solve this problem, scientists have invented blood collection tubes containing anticoagulants, among which the purple EDTA tube is specifically designed for this purpose.
EDTA, Ethylene diamine tetraacetic acid (EDTA) is a calcium ion chelating agent that can prevent blood clotting and maintain sample integrity. The K2 EDTA powder in the purple EDTA tube is evenly distributed on the tube wall. When blood is collected into the tube, EDTA quickly binds to calcium ions in the blood, preventing the formation of blood clots and creating conditions for subsequent DNA extraction.
3、 Collection process
The correct collection of blood samples is crucial for the accuracy of paternity testing. Collectors should ensure that all instruments are in a sterile state, clean the blood collection site with alcohol swabs, and reduce the risk of infection. Subsequently, using specialized needles and purple EDTA tubes, blood was drawn from the subject's elbow vein or other suitable location.
After the collection is completed, the blood collection tube should be immediately reversed several times to fully mix the blood with EDTA and ensure anticoagulant effect. In addition, to maintain sample activity, exposure to high temperatures should be avoided, especially during the hot summer months. Samples should be transported under low temperature conditions, such as using specific containers with ice packs.
4、 Sample storage and transportation
Proper storage of samples is equally important. The collected blood samples should be stored separately to avoid confusion with other samples. At the same time, the identity information of the subjects, including name, sample type, and collection date, should be clearly marked on the blood collection tube to ensure the accuracy of subsequent analysis.
During transportation, the sample should be kept at a low temperature to prevent DNA degradation. Using insulated containers with ice cubes for transportation can maintain the stability of the sample until it is received and further processed in the laboratory.
The application of purple EDTA tubes in paternity testing reflects the combination of scientific rigor and technological progress. By using these specially designed blood collection tubes, not only has the blood sample collection process been simplified, but the quality of the samples and the accuracy of the identification results have also been greatly improved. In the future, with the continuous advancement of technology, the technology of paternity testing will become more mature, providing society with more fair and accurate proof of blood relationship.
By delving into the application of purple EDTA tubes in paternity testing, we not only see the important role of science and technology in modern society, but also recognize the responsibility and mission behind every scientific tool. In every detail of paternity testing, there is a respect for the dignity of life and a persistent pursuit of truth. Choosing Desheng as your supplier of ethylenediaminetetraacetic acid dipotassium and tripotassium salts will provide you with high-quality products, professional services, and reliable guarantees. If you have relevant purchasing intentions, please click on the website to inquire about details!