Serum Separation Gel Wall Hanging: Phenomena, Causes, and Response Strategies

Serum separation gel wall hanging is one of the common problems in the laboratory, which not only affects the experimental results but may also cause difficulties in the experimental process. To effectively address this issue, we need to have a deep understanding of the reasons for serum separation gel hanging on the wall, and based on this, propose targeted solutions.

Cause analysis

The main reasons for serum separation gel hanging on the wall include:

1. The high content of fibrinogen in serum leads to the formation of a network structure during centrifugation, which is easily adsorbed on the centrifuge tube wall and serum separation gel.

2. Quality issues with serum separation gel, such as insufficient viscosity or improper composition ratio, resulting in excessive binding force with the test tube wall.

3. The rotation speed and time during centrifugation are set improperly, resulting in incomplete separation of serum or excessive foam.

4. Improper handling of serum samples, such as insufficient mixing or high concentration of particles.

processing method

For the above reasons, the following are some effective handling methods:

1. Choose high-quality serum separation gel: When purchasing serum separation gel, you should choose a certified brand and manufacturer, and carefully check the product description and ingredient list. Avoid using serum separation gel with insufficient viscosity or improper composition ratio.

2. Optimize centrifugation conditions: Based on the characteristics of serum samples and experimental requirements, set the centrifugation speed and time reasonably. Generally speaking, the centrifugal speed should not be too high and the time should not be too long to avoid the generation of foam in the serum and excessive vibration of the centrifuge tube. By optimizing the centrifugation conditions, the phenomenon of serum separation gel hanging on the wall can be reduced.

3. Regular cleaning and maintenance of the centrifuge: The centrifuge is prone to accumulate dirt and residues during use. If not cleaned and maintained in a timely manner, it can affect the centrifugal effect and the accuracy of experimental results. Regularly cleaning the inner chamber and rotary head of the centrifuge, inspecting and replacing the sealing ring of the rotary head and other accessories, can maintain the good performance of the centrifuge and extend its service life.

4. Use auxiliary tools: There are some auxiliary tools on the market that can help solve the problem of serum separation gel hanging on the wall. For example, centrifuge tube supports can reduce the degree of tube shaking during the centrifuge process, thereby reducing wall hanging phenomenon. In addition, some manufacturers have also launched specialized tools or equipment for removing serum separation gel, which can be selected and used according to needs.

5. Operation skills: The correct operation method can to some extent reduce the phenomenon of serum separation gel hanging on the wall.

Summary and suggestions

In summary, to effectively address the issue of serum separation gel hanging on the wall, it is necessary to start from multiple aspects. Firstly, selecting high-quality serum separation gel is the foundation; Secondly, optimizing centrifugal conditions and improving the maintenance level of centrifuges are important measures; The use of auxiliary tools and manual operation skills is also a feasible solution. In practical operation, appropriate methods can be selected according to specific situations for processing. At the same time, in order to improve experimental efficiency and accuracy, it is recommended that laboratory staff strengthen communication and collaboration, and form a complete and standardized operating process and management system.

Hubei Xindesheng Material Technology Co., Ltd., as a professional chemical reagent manufacturer, has always been committed to providing high-quality and high-purity chemical reagents, using advanced production equipment and complete production lines. At present, the company has a complete range of additives for blood collection vessels produced and sold. If you are interested, please feel free to contact us for purchase at any time!