Sodium citrate tube can be used for coagulation test

Among the numerous examination items in medical laboratories, coagulation test, also known as coagulation function test or coagulation four item test, is an indispensable part of evaluating patients' coagulation status and diagnosing hemorrhagic and thrombotic diseases. In this precise medical testing, sodium citrate tubes play a crucial role as containers for collecting blood samples. This article will delve into the application, advantages, and key factors ensuring the accuracy of sodium citrate tubes in coagulation tests.

Sodium citrate tube action

Sodium citrate tube, as the name suggests, contains a specific concentration of sodium citrate solution as an anticoagulant inside. Sodium citrate plays an irreplaceable role in coagulation tests due to its anticoagulant mechanism. It combines with calcium ions in the blood to form soluble complexes that are difficult to dissociate, thereby significantly reducing the concentration of free calcium ions. This process inhibits the conversion of prothrombin to thrombin, blocks the coagulation cascade reaction, and keeps the blood in a liquid state after collection, avoiding the occurrence of natural coagulation.

The importance of blood coagulation examination

The coagulation test mainly includes the determination of prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), and fibrinogen (FIB). These indicators can comprehensively reflect the coagulation function status of the body, and are of great significance for the diagnosis, monitoring, and development of treatment plans for various diseases.

Advantages of Sodium Citrate Tube

In coagulation tests, sodium citrate tubes have significant advantages compared to other anticoagulant tubes such as EDTA tubes, heparin tubes, etc. Sodium citrate has a good protective effect on coagulation factor five, avoiding the decrease or loss of coagulation factor activity caused by anticoagulant effects, thereby ensuring the accuracy of test results. Secondly, the anticoagulant effect of sodium citrate is moderate, neither excessively diluting the blood sample nor affecting the natural sedimentation of red blood cells, which is beneficial for maintaining the physiological state of the blood sample. In addition, sodium citrate also has good stability and compatibility, and can cooperate well with various detection reagents and instruments, improving detection efficiency.

Key factors ensuring detection accuracy

The key factors to ensure the accuracy of coagulation tests using sodium citrate tubes include:

1. The ratio of anticoagulant to blood: The requirement for the ratio of anticoagulant to blood must be strictly followed, generally 1:9 (i.e. adding 1 milliliter of sodium citrate solution to every 9 milliliters of blood). For patients with abnormal hematocrit, the amount of anticoagulant should be adjusted according to the formula to ensure moderate anticoagulant effect.

2. Blood collection operation standards: During blood collection, excessive force or blood contamination should be avoided to avoid damaging red blood cells or introducing exogenous coagulation factors, which may affect the test results.

3. Timely sample processing: The collected blood sample should be sent to the laboratory for testing as soon as possible to avoid prolonged storage that may cause degradation of coagulation factors or sedimentation of red blood cells.

4. Testing instruments and reagents: Choose high-quality testing instruments and reagents to ensure the stability and reliability of the test results.

5. Quality control: Establish a comprehensive quality control system, regularly calibrate and maintain testing instruments, verify testing reagents, and ensure the accuracy and traceability of testing results.

In summary, sodium citrate tubes play an irreplaceable role in coagulation tests. Its anticoagulant mechanism, good protective effect on coagulation factors, and good compatibility with other detection systems make sodium citrate tubes an indispensable tool in coagulation tests. By strictly following the operating procedures, ensuring the appropriate ratio of anticoagulants to blood, processing samples in a timely manner, and selecting high-quality testing instruments and reagents, the accuracy and reliability of coagulation tests can be further improved, providing stronger support for clinical diagnosis and treatment. Sodium citrate is one of the main products of Hubei Xindesheng Company, which stands out in the market for its high purity, good water solubility, and excellent efficacy, and is highly praised by domestic and foreign customers. Complete variety, welcome to purchase!