When it comes to chemiluminescence or acridinium esters, you may think that it is a term that is too professional, far away from your life, even in the university's biochemistry major, it is rarely mentioned.However, if I say immune response, you are probably no stranger. Chemiluminescence immunoassay (chemiluminescence immunoassay, CLIA) started in the early 1980s and rapidly developed in the 1990s, becoming a development after fluorescence immunoassay, radioimmunoassay and enzyme-linked immunoassay Emerging immunodetection technology. It uses the specific reaction of antigen and antibody for detection, and uses isotopes, enzymes, chemiluminescent substances to amplify and display the detection signal, and is often used to detect trace substances such as proteins and hormones. In connection with actual life, that is, you go to the hospital to check the thyroid hormones, the diagnostic methods that sex hormones will use.
Immunological diagnosis occupies a very important position in clinical diagnosis. Common immunological techniques include radioimmunity, enzyme-linked immunity, chemiluminescence, electrochemiluminescence, and nanomagnetic particle chemiluminescence. Chemiluminescence immunoassay technology mainly has the advantages of high sensitivity, strong specificity, good reagent stability and long validity, wide detection linear range, simple operation, stable and fast method, many test items (as shown below), and high degree of automation. It has become the mainstream method of immunoassay technology and is widely used in various fields of clinical testing.
Acridinium ester labeled antibody detection IgG
The acridinium ester mentioned above is a type of chemical substance that can be used as a chemiluminescent marker. In alkaline H2O2 solution, when the molecule of the acridinium ester is attacked by hydrogen peroxide ions, the substituent on the acridinium ring can It forms unstable dioxyethane with C-9 and H2O2 (hydrogen peroxide) on the acridinium ring, which decomposes into CO2 and electronically excited N-methylacridone. There are a total of 7 different groups of acridinium ester products, 1. AE-NHS (traditional acridinium ester); 2. DMAE-NHS; 3. Me-DMAE-NHS; 4. NSP-DMAE-NHS; 5 . NSP-SA-NHS; 6. NSP-SA; 7. NSP-SA-ADH.
These acridinium esters produced by Desheng have the characteristics of high product purity and small batch-to-batch difference. They also provide other immune reagents for matching use, provide a full set of technical services and technical support, please contact us for inquiries! Website: www.vacutaineradditives.com, Phone: +86-711-3702650.
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