THAM, or tris(hydroxymethyl)aminomethane, is a weak base and is often used as a biological buffer; hydrochloric acid and sulfuric acid are two essential acids in the laboratory, but concentrated sulfuric acid is very easy to absorb water, concentrated hydrochloric acid is easy to volatile, and their concentration is not Stable, usually calibrated by soda ash (sodium carbonate); now it has been found that it is more advantageous to use THAM instead of soda ash to calibrate the acid concentration in potentiometric titration.
The significance of titrating acid concentration:
In many experiments, the required acid (hydrochloric acid, sulfuric acid) concentration is different, or sometimes it is necessary to use different concentrations of acid at the same time. At this time, in order to ensure the exact concentration, it is necessary to titrate with sodium carbonate. The price of sodium carbonate is relatively cheap, but the high-purity sodium carbonate reference substance is easy to absorb moisture, and some of it will be converted into sodium bicarbonate (baking soda) to affect the titration. Need high temperature treatment and need to boil and then cool before the end of titration. This process requires manual titration, which is not conducive to automatic potentiometric titration.
THAM Tris(hydroxymethyl)aminomethane reagent
Advantages of THAM Potentiometric Titration:
Manual titration is actually easy to cause errors and poor repeatability. For example, the personnel operation requirements are very high, the analysis steps are relatively large, and because it is judged by the color change that different people will have differences. Modern potentiometric titration is different. It does not require people to judge by color change, only the instrument records the potential mutation point.
Potentiometric titration of acid with soda ash can eliminate the boiling and cooling steps before the end point. It is more accurate to judge the end point by the potential mutation point than when the indicator changes color. The disadvantage is that the potential mutation point of sodium carbonate is small, and there are multiple mutation points before the end point that are not conducive to judging the end point (caused by not heating before the end point). Using THAM instead of soda ash potentiometric titration, the obtained potential jump is sharp and single, the titration end point is obvious, and the calibration result is consistent with soda ash, and there is no other influence.
THAM potentiometric titration calibration of acid concentration is not only used to calibrate the concentration of hydrochloric acid and sulfuric acid, but also applicable to other acids. While the automatic potentiometric titration data is consistent with the results of manual titration indicator method, it also reflects the simplicity of instrument analysis and operation. Heating like soda ash titration, faster efficiency and good parallelism. Desheng is a specialized manufacturer of biochemical reagents, which can supply large quantities of high-quality THAM reagent raw materials.
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