The advantages of acridine ester luminescent system compared with luminol system

Acridine ester, a yellow powder, CAS No.: 194357-64-7, has high quantum yield and high chemiluminescence efficiency, which is five times or more than that of luminol. The chemiluminescence process of acridine ester is rapid, with low background, and can emit light in the presence of sodium hydroxide and hydrogen peroxide. It is one of the most widely used chemiluminescence markers and plays an important role in chemiluminescence immunoassay.

Acridine ester

Chemiluminescence immunoassay (CLIA) is a combination of highly sensitive chemiluminescence assay and highly specific immune reaction, which is used for the detection and analysis of various antigens, hapten, antibodies, hormones, enzymes, fatty acids, vitamins and drugs. There are many kinds of chemiluminescence systems used in clinical examination, the most important are luminol and its derivatives, acridine ester, spiroamantane-1,2-dioxane and its derivatives, and electrochemiluminescence system.

Among various chemiluminescence systems, acridine esters are considered to be ideal substrates. What are the unique advantages of acridine esters compared with luminol system?

1. The luminescence system of acridine ester is simple and no catalyst is needed.

Under alkaline conditions, acridine ester molecules can be attacked by hydrogen peroxide to produce dioxane, which is unstable and decomposes into CO2 and n-methylacridone in electronic excited state. When it returns to the ground state, it emits light with a wavelength of 430nm without catalyst, and the luminescence system is simple.

2. Acridine ester has high luminous efficiency and intensity.

Acridine ester chemiluminescence is a flash type, which has advantages over other techniques in the field of chemiluminescence immunoassay. The intensity of the chemiluminescence reaches the maximum after 0.4 s, the half-life is 0.9 s, and the luminescence basically ends within 2 s, which can realize rapid detection. Acridine ester has high quantum yield and high chemiluminescence efficiency, It's usually five times or more than luminol.

3. Acridine ester luminescence system has few interference factors, low background and high signal-to-noise ratio.

The characteristic of acridine esters from the perspective of luminescence mechanism is that the non luminescent substituents attached to the acridine ring are separated from the acridine ring before the formation of electron excited state intermediates, that is, the non luminescent part is separated from the luminescent part, so the luminescence efficiency is not affected by the substituent structure.

In addition, acridine ester has small molecular weight, easy to bind with protein through chemical bond (i.e. labeling), little influence on the conformation of antibody after conjugation (thus ensuring its good reactivity in subsequent immune detection), and good stability of conjugates.

Desheng has six kinds of acridine ester products with different groups: acridine ester dmae-nhs, acridine ester nsp-dmae-nhs, acridine salt nsp-sa-nhs, acridine salt nsp-sa-nhs, acridine hydrazide nsp-sa-adh, acridine ester me-dmae-nhs.

Desheng is an old chemical reagent company specializing in the R & D and production of blood test reagents. Up to now, it has 14 years of R & D and production experience. Luminescent reagent substrate has always been the top priority of the company's research. Acridine ester series has high luminous efficiency and belongs to direct luminescence. Need acridine ester series of business friends can directly contact 18971041571 (wechat number), looking forward to your call!