The chemical name of HEPES is 4-hydroxyethyl piperazine ethanesulfonic acid, cas7365-45-9. HEPES is commonly used in biological buffers, cosmetics, coatings, surfactants, etc. The pH buffer range is 6.8-8.2, which can be used in biochemical diagnostic kit, DNA / RNA extraction kit and PCR diagnostic kit. It has no toxic effect on cells. It is a hydrogen ion buffer, which can control the constant pH range for a long time.
HEPES buffer is often used in cell culture experiments. In the open culture condition, the addition of HEPES buffer can prevent the increase of pH oxidation in the medium, thus maintaining the pH at about 7.0. So, what are the preparation steps and methods of HEPES buffer? Let's introduce them in detail by Desheng.
Different literatures have different opinions on the preparation of HEPES buffer solution. According to the use, one is pure HEPES + NaOH, the other is HEPES + salt. Various preparation methods are summarized as follows:
1、 Preparation of 500 ml 1 m HEPES, pH = 7.0, stock solution
Dissolve 119.15 g HEPES in 400ml distilled water, add 0.5 ~ 1m NaOH aqueous solution to adjust at least the required pH (the effective pH range of HEPES is 6.8 ~ 8.2), then fix the volume to 500ml with distilled water and store at 4 ℃.
2、 HEPES buffer formula with small amount of salt (500ml)
HEPES 6.5g, NaCl 8.0g, na2hpo4.7h2o 0.198g, adjust the pH value with 0.5m NaOH solution, and finally fix the volume.
3、 Preparation of 2 × HEPES buffer salt solution
Dissolve 1.6g NaCl, 0.074g KCl, 0.027g na2hpo4.2h2o, 0.2g dextran and 1g HEPES in 90ml distilled water, adjust to the required pH value with 0.5m NaOH, and then fix the volume to 100ml with distilled water.
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Desheng's main product biological buffer is a kind of alcohol ammonia fine chemicals with special characteristics. It does not participate in or interfere with the biochemical process. It can be used in the buffer system of life science research and provide a stable pH environment for experiments. Desheng's biological buffer is widely used in in vitro diagnosis, biopharmaceutical, biological beauty, paint and other industries because of its high buffering efficiency and high quality There are dozens of stable customers. Desheng provides one-stop purchasing service. If necessary, you can click the official website to consult customer service.
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