Bicine buffer, dihydroxyethylglycine, CAS No. 150-25-4, is a biological buffer with a wide range of uses. Like Tris, it has superior buffering performance and is gradually replaced in some demanding biochemical tests Traditional phosphate buffer.
Bicine is an amino acid derivative with weak acidity, pH buffer range of 7.6-9.0, soluble in water and commonly used organic solvents such as DMF, DMSO, DMAc, etc. When the powdered bicine is formulated into a solution, sodium hydroxide is usually added to adjust the pH value, so as to meet the pH environment requirements required for experiment or detection. If the experimental environment needs to eliminate the interference of sodium ions, potassium hydroxide can be used instead of sodium hydroxide, and then the pH can be measured by a pH meter to adjust the mixing ratio of the Bicine solution and the lye.
Similar to the working principle of other biological buffers, Bicine is amphoteric. When acid and alkali are added to the reaction system or the pH of the reaction environment changes as the reaction progresses, it can act as a buffer to maintain the reaction system. pH value, which is intuitively important for reactions involving enzymes or proteins and accounting in biochemical detection. The stability and functionality of most organic biomolecules are very sensitive to pH.
The molecular structure of Bicne is similar to glycine. The hydrogen on the amino nitrogen atom is replaced by two hydroxyethyl groups, which reduces the hydrolysis ability of the amino terminal and enhances its stability. At the same time, it has the properties of amino acids and aminoethanol, and can form a metal complex with a single anion Thing. The buffer system configured with Bicine and sodium hydroxide will not participate in the chemical reaction of the reaction system, nor interfere with the experimental reaction, and is resistant to chemical and enzymatic hydrolysis.
Desheng is a manufacturer specializing in biochemical testing, in vitro diagnostic raw material production and research and development. It has rich experience in blood pretreatment, blood pretreatment and biochemical kits. At present, the mature product series include blood collection tube additives, chromogenic reagents, chemiluminescence Reagents and biological buffers including more than ten Good's reagents such as Bcine.
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