There is a way to make the luminol luminescence system enhance a lot
Luminol is also called luminol. Its CAS number is 521-31-3. Its appearance is a yellow powder. It is a chemiluminescent reagent. Chemiluminescence has the characteristics of high sensitivity, wide detection linear range and no pollution. It is an ideal immunoassay technique. There is a way to increase the luminescence of luminol a lot. In chemiimmunoluminescence analysis, the use of catalysts and luminescence enhancers can stabilize the chemiluminescence, increase the intensity and prolong the luminescence time. In addition, constructing a catalytic interface that can preferentially attract reactants is also one of the important strategies to enhance chemiluminescence.
It can be seen in the literature that academician Wang Erkang and associate researcher Li Jing of Changchun Institute of Applied Chemistry of the Chinese Academy of Sciences used in-situ synthesis to construct a flower-like cobalt hydroxide catalytic interface with a layered porous structure in an environment where BSA and Co2+ coexist. Effectively enhance chemiluminescence.
Cobalt hydroxide itself has unique physical and chemical properties, its layered structure and good electrochemical oxidation-reduction performance, has a wide range of applications in catalysis, electrochemistry, gas-phase sensors and haloacetic acid degradation, etc., and cobalt hydroxide is excellent The characteristics are affected by its morphology and size. This flower-like cobalt hydroxide catalytic interface with a layered porous structure has oxidase activity and a unique microstructure, which can effectively generate active oxygen.
At present, chemiluminescence immunoassay mainly includes luminol, isoluminol, acridine and cyclodioxane derivatives and other systems. The researchers tested the effect of this catalytic interface on the luminescence intensity of the luminol luminescence system mediated by BSA and Co2+ under alkaline conditions. The results show that compared with the traditional luminol-Co2+ or luminol-BSA system, the in-situ catalytic interface can achieve a 100-fold increase in chemiluminescence.
In addition, this catalytic enhancement mechanism can also be extended to different proteins, such as lysozyme, protamine, thrombin, and papain. The researchers also built a sensing platform that can measure ascorbic acid based on the quenching effect of chemiluminescence, and proved its good performance.
After the above, this research work provides a new way to design a catalytic interface that can enhance CL luminescence. It can help the chemiluminescence system to play a role in inorganic analysis, organic analysis, immunoassay, nucleic acid analysis and other fields. Hubei Xindesheng Materials also produces luminol, isoluminol, acridine esters and other chemiluminescence reagents. Among them, luminol has been researched and produced for many years. The product purity is ≥98% and the chemiluminescence efficiency is high. The products produced by Hubei Xindesheng Materials are of high quality and preferential prices. If you want to know, you can call to discuss in detail.
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