The chromogenic substrate ADOS is a raw material used for chromogenic in the biochemical kit, with CAS No.82692-96-4, which can be oxidized with 4-AAP by hydrogen peroxide in the presence of peroxidase to produce chromogenic reaction, which is sensitive, rapid detection. Here is a guide for using the ADOS reagent produced by our company for reference only.
Appearance and packaging of ADOS
The packaging is a foam insulation box, containing blue ice or ice packs. The product manual and test report are included. If it is not complete, please call customer service to request an electronic version. Packed in dark brown bottles, the reagent is white crystal powder, if the color is deepened, it may have grown bacteria or partially oxidized and cannot be used.
After receiving the goods, will the product deteriorate if the built-in ice pack has melted?
This product is stable at room temperature. Low temperature transportation is to prevent extreme temperature conditions that may occur during transportation. Therefore, when you receive the product, if the ice pack has been melted, it will not affect its quality, please feel free to use. If it needs to be stored for a long time, it is recommended to store it in freezing and dark. If it has been prepared into a solution, it is recommended to use it immediately to avoid oxidation and discoloration in contact with air.
Configuration of ADOS solution
The chromogenic substrate ADOS is a highly water-soluble aniline sodium salt derivative improved on the basis of traditional chromogen phenol and aniline. Reagent powder may adhere to the wall of the tube or bottle, allowing centrifuges to be centrifuged at low speed to reduce product loss; the product has good solubility and can be directly dissolved in deionized water; double-distilled water or super Pure water; when special circumstances require DMSO as a solvent, first check its solubility in DMSO, and set the corresponding DMSO concentration control group; in special cases, use water bath or ultrasonic vibration to assist dissolution; the concentration changes too quickly during the dilution process It can be reconstituted by ultrasound.
The product is sterile or not
This product is a powder reagent. It is frozen and preserved, which reduces the possibility of bacteria growth of solution reagent. When preparing the solution, only keep the operating environment and equipment sterile. If strict sterilization is needed, it is recommended to use a bacterial filter instead of high temperature and high pressure sterilization.
When ADOS is used as a chromogenic substrate to participate in the hydrogen peroxide coupling chromogenic reaction, it requires the participation of peroxidase. Therefore, the pH of the reaction system is strict. It is recommended to use a biological buffer produced by Desheng Technology to adjust the reaction; Environmental pH ensures enzyme activity and improves detection sensitivity.
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