High-quality Tris buffer is an important part of electrophoresis. It allows current to pass through the sample while resisting changes in the pH value of the entire solution. The choice of buffer depends on the isoelectric point of the sample being analyzed. In DNA electrophoresis, the commonly used buffers are Tris-Acetate-EDTA and Tris-Borate-EDTA. In protein electrophoresis, SDS (sodium dodecyl sulfate) is usually used. These buffers help separate samples into readable gels. Depending on the sample, other buffers can help improve results or expand readings at specific molecular weights.
Recently, I have obtained some knowledge about electrophoresis buffer through professional literature, and I want to share and discuss with you here.
The role of Tris-acetate-EDTA and Tris-borate-EDTA in agarose gel electrophoresis:
Tris is a strong base and boric acid is an acid. The combination of the two can keep the pH in the neutral range of 8 to 8.5. Under such alkaline conditions, DNA is protected and can be separated properly. EDTA plays an important role in this combination. EDTA is a chelating agent. It chelates the Mg2+ ions required by the DNAse enzyme as a cofactor. DNAse is an enzyme that cuts DNA into small fragments. Therefore, by adding EDTA, we can protect our DNA from enzymatic activity. In addition, the buffer will neutralize the charge of the water molecules. Under the action of electric current, water molecules decompose into H + and OH-. H + ions can react with the PO3- of DNA. Therefore, the negative charge of the buffer will neutralize the charge in the water and protect the DNA.
Although Tris-Acetate-EDTA and Tris-Borate-EDTA have the same role in electrophoresis, they have their own advantages and disadvantages. Tris-acetate-EDT buffer can interfere with enzymatic reactions and protect DNA, while Tris-borate-EDTA buffer cannot.
In summary, the use of Tris-acetate-EDTA and Tris-borate-EDTA derived from Tris buffer in electrophoresis is very important, and this is one of the reasons why Tris buffer is in short supply in today’s market. One. Last time now, it was very difficult to find a manufacturer specializing in the production of Tris, but here I would like to recommend our company, Hubei Xindesheng Materials Co., Ltd.
Hubei Xindesheng Material Co., Ltd. specializes in the production of Tris, Hepes, Mops and other buffer products. Up to now, the company has been established for decades and has very rich experience in the development and production of biological buffers. We can provide a large number of Tris and other types of biological buffer products, and our company can provide product customization and professional after-sales service for customers, and provide professional product sample testing services and packaging customization services for special customers. In any case, Desheng Company must be your best choice for purchasing biological buffers.
