CAPS, also known as 3-cyclohexylaminopropanesulfonic acid, is a biological buffer with white crystals powder in appearance. CAPS can be dissolved in water and the pH buffer range is 8.9-10.3.Its PKA value is 9.6 when the temperature is below 25°C, and the molecular weight is 243.28.The CAS number OF CAPS is 1135-40-6.What Are CAPS Buffer Can Be Applied In?Actually, CAPS has a wide range of applications, including DNA/RNA extraction kits, PCR diagnostic kits, biochemical diagnostic kits, manufacturing welding materials, air conditioning equipment, manufacturing metal lithium, new coatings, and new industrial materials.
In nucleic acid extraction, it can be combined with 3-[(3-cholamidoendyl)dimethylammonium]-1-propanesulfonate (CHAPS), 3-(cyclohexylamino)-2-hydroxy-1- propanesulfonate (CAPSO), and 2-(cyclohexylamino)ethanesulfonate (CHES),together to prepare a solution for nucleic acid hybridization. It can reduce the yield of non-specific hybrids and improve the specificity of nucleic acid hybridization, while maintaining the yield of target hybrid products, which is of great value in the identification of specific pathogens, the diagnosis of genetic disorders, and the analysis of DNA sequences.
CAPS can be used in various environmentally friendly and high-quality waterborne component polyurethane coatings, and can coexist stably with resins containing active DNA (hydroxyl, carboxyl, amine, epoxy, etc.) for a long time at room temperature. The application of CAPS in coatings is widely used by the waterborne coatings market. In addition, CAPS and aliphatic polyisocyanates can react in the presence of tertiary amine neutralizing agents under mild conditions. The resulting sulfonic acid urea derivatives are very good emulsifiers.
CAPS can be used to prepare the running buffer (also known as electrolyte solution) in capillary electrophoresis, and as the end electrolyte to make the electrokinetic pressurized online enrichment and separation of 2-nitrobenzoic acid and 3-nitrobenzoic acid. The CAPS buffer is suitable for running buffers of high molecular weight proteins (greater than 20KD). If it is a low molecular weight protein blotting experiment, combined Tris with Glycine is generally used, and if glycine is excluded by protein sequencing, Tris-Tricine and EDTA is used.
CAPS can also be used in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits, as buffers for enzymatic chemistry and HPLC separation of alkaline drugs. CAPS can be used as a western blot transfer buffer or transfer buffer in the transfer process of protein sequencing. CAPS can also support alkaline phosphatase activity and suppress the growth of aeromonas at pH 105.After dissolving in deionized water and adjusting the pH to 110 , it was used for the purification of fibronectin. In addition, CAPS is also used as an eluent in cation exchange chromatography,as a raw material in manufacturing welding materials for air conditioning equipment. What’s more,It can also be used to make fireworks, used as analytical reagents, heat exchange carriers,etc.
Desheng have been developing and producing biological buffers for many years with rich experience.CAPS is one of our key products sold well in the market,inquiries are welcome if you have any requirements.
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