The full name of Tris-Hcl in Chinese is Tris Hydrochloride, another name is Tris Hydrochloride, and the English name is TRIS hydrochloride. I believe that many people are familiar with Tris. Tris has a wide range of applications. Tris buffer is not only used as a solvent for nucleic acids and proteins, but also has many important uses.
Tris is used for protein crystal growth under different pH conditions. The low ionic strength of Tris buffer can be used for the formation of the intermediate fibers of the nematode nuclear laminin. Tris is also one of the main components of protein electrophoresis buffer. It forms a buffer system with glycine in the electrophoresis buffer to stabilize the pH during electrophoresis. In addition, Tris is also an intermediate for the preparation of surfactants, vulcanization accelerators and some drugs. Tris is also used as a titration standard. As a protein buffer, if mass spectrometry is required for subsequent work, Tris is not suitable. Try to replace it with a buffer that can be tolerated by other mass spectrometers.
Tris-HCl is derived from the reaction of Tris as the main raw material. Its solution is acidic and is mainly used as a biological buffer to adjust the pH of the virus preservation solution. If you need to adjust to neutral or alkaline, you can add Tris or NaOH to achieve. In addition to this information, what are the configuration methods for Tris-Hcl? How much do you know? The configuration method of Tris-Hcl will be introduced below.
Preparation method of Tris-HCl buffer:
1. Tris-Hcl (0.05mol/L, 25℃)
After mixing 50ml 0.1mol/L tris (Tris) solution with x ml 0.1mol/L hydrochloric acid, add water to dilute to 100ml
PH x/ml PH x/ml
7.1 45.7 8.1 26.2
7.2 44.7 8.2 22.9
7.3 43.4 8.3 19.9
7.4 42.0 8.4 17.2
7.5 40.3 8.5 14.7
7.6 38.5 8.6 12.4
7.7 36.6 8.7 10.3
7.8 34.5 8.8 8.5
7.9 32.0 8.9 7.0
Tris molecular weight=121.14: 0.1mol/L solution is 12.114g/L. Tris solution can absorb carbon dioxide from the air, so the bottle cap should be tightly closed when using. When configuring Tris-Hcl solutions with different concentrations and different pH values, you can first prepare a 0.05mol/L solution with a certain pH value, and then dilute to the required concentration.
2. 1M Tris-Hcl (PH7.4, PH7.6, PH8.0)
Component concentration 1M Tris-Hcl
Configuration volume 1L
Configuration method:
1. Weigh 121.1g Tris and place it in a 1 L beaker;
2. Add about 800ml of deionized water, fully stir to dissolve;
3. Add the required PH value for the adjustment of the concentrated HCL amount according to the following value;
PH value Concentrated HCL
7.4 Approximately 70ml
7.6 about 60ml
8.0 about 42ml
4. Dilute the solution to 1L;
5. Store at room temperature after autoclaving.
3. 1.5M Tris-Hcl (PH8.8)
Component concentration 1.5M Tris-Hcl
Configuration volume 1L
Configuration method:
1. Weigh 181.7g Tris in a 1L beaker;
2. Add about 800ml of deionized water, fully stir to dissolve;
3. Adjust the PH value to 8.8 with concentrated Hcl;
4. Dilute the solution to 1L;
5. Store at room temperature after autoclaving.
It should be noted that the solution should be cooled to room temperature before adjusting the pH value, because the pH value of the Tris solution varies greatly with temperature, and the pH value of the solution decreases by approximately 0.03 units for every 1°C increase in temperature.
The above is the configuration method of Tris-Hcl provided by Hubei Xindesheng Material Co., Ltd. In addition to the production of Tris-Hcl, Desheng also produces other chemicals, such as blood collection tube additives, enzyme preparations, chemiluminescence reagents, biological buffers, chromogen substrates and carbomer products. At present, Desheng company has been established for decades, has its own R&D team, and has invested a lot of energy in biological buffers. The quality of the products is reliable, the price is favorable, and if you are interested in understanding, you can call for consultation. Desheng welcomes your calls.
