What is the purity test method of potassium ethylenediaminetetraacetate?

What is the purity test method of potassium ethylenediaminetetraacetate?

Bottled EDTA dipotassium ethylenediaminetetraacetic acid dipotassium is called EDTA-2K for short. As an anticoagulant commonly used in blood collection vessels, its anticoagulation performance is good, so it is favored by many downstream reagent manufacturers and medical institutions. At the same time, there are more and more factories producing potassium ethylenediaminetetraacetate in the upstream, the quality becomes uneven, and many inferior products are mixed in.

The so-called "inferior" products refer to unstable performance, low purity and appearance defects. Among so many products with poor quality, how should buyers identify them?

The key to determine the performance of potassium ethylenediaminetetraacetate is its purity, pH value, clarity, solubility and impurities. Therefore, special attention should be paid to these aspects, and testing can be conducted if necessary to avoid purchasing products with poor performance. So, what is the purity test method of potassium ethylenediaminetetraacetate? Next, I would like to give you a detailed introduction.

1. Purity test

Weigh 0.1g of EDTA potassium sample, accurate to 0.0001g if necessary, add 70mL of water and 10mL of ammonia ammonium chloride buffer solution with pH value of about 10. After the sample is fully dissolved, add 5 drops of chrome black T indicator, 5g/L, and titrate with zinc chloride standard titration solution until the solution turns red purple [c (ZnCL2)=0.025mol/L].

The purity of potassium ethylenediaminetetraacetate produced by Desheng is higher than 100, which is a normal phenomenon, because the company's potassium ethylenediaminetetraacetate is a dihydrate, and the main content detected contains two crystal water.

2. PH value test

Weigh 5g of test potassium sample, dissolve it in 100ml of pure water, stir for about 5min at room temperature, and test the PH value. The pH value of Desheng's potassium is 4.8 ± 1 (5% aqueous solution, g/ml).

3. Clarity test

Weigh 20g of test sample, dissolve it in 100ml of pure water solution, stir it for 5-10 minutes at room temperature, and compare the prepared water solution with colorless transparent liquid.

4. Solubility test

Weigh 60.0g of the sample to be tested, dissolve it in 100ml of pure water, stir for 5-10 minutes at room temperature (25 ± 5 ℃), and observe the dissolution.

5. Impurity test

Place 0.22 filter membrane in the testing vessel, pour the solution into the filter, and observe whether there are impurities on the filter membrane and whether there are too many impurities.

The above five aspects are all methods to test the performance of potassium ethylenediaminetetraacetate. If the test is up to the standard, the performance is stable and will not interfere with the test. Desheng Biochemical has been committed to developing additives for blood collection vessels. One of the main products is potassium ethylenediaminetetraacetate, which is not only 99% pure, but also has strict requirements in all aspects. Its performance is extremely stable. If you have a demand for potassium ethylenediaminetetraacetate, please click the website to consult and buy!