Tris, CAS: 77-86-1. It is a white crystal or powder, soluble in ethanol and water, slightly soluble in ethyl acetate and benzene, corrosive to copper and aluminum. Tris has high buffer capacity, high solubility in water and is inert to many enzyme reactions, which makes Tris a very satisfactory buffer for many biochemical purposes. It is used to stabilize the pH of the reaction system and has a strong buffer capacity between pH 7.5 and 9.0.
Desheng Tris packaging
Tris in glyphic acid buffer system was used to stabilize pH value in the electrophoretic buffer solution. The Tris-HCl buffer system was used to stabilize pH value in the gel. It was widely used as a solvent for nucleic acids and proteins. The low ionic strength of Tris buffer could also be applied to the formation of intermediate fibers of nematodes. EDTA buffer was added into Tris hydrochloric buffer to make TE buffer, which could be used for the stabilization and storage of DNA. The "Tae buffer" can be obtained by replacing the acid solution with acetic acid, and tbe buffer can be obtained by replacing it with boric acid. These two buffers are often used in nucleic acid electrophoresis experiments.
Tris is used in both TAE and tbe buffer (for nucleic acid dissolution) in biochemical experiments. It contains amino groups and can react with aldehydes. Tris is a weak base and its PKA is 8.1 at 25 ℃. The effective buffer range of Tris buffer is between pH 7.0 and 9.2.
The pH value of Tris base aqueous solution is about 10.5. Generally, hydrochloric acid is added to adjust the pH value to the desired value to obtain the buffer solution with this pH value. At the same time, we should pay attention to the effect of temperature on pKa of Tris. Because Tris buffer is a weak alkaline solution, DNA will be deprotonated in such a solution to improve its solubility.
People often add EDTA into Tris hydrochloric acid buffer to make "Te buffer", which is used for DNA stabilization and storage. If the acid solution of adjusting pH value is replaced by acetic acid, the "Tae buffer" (Tris / acetate / EDTA) is obtained, and the "tbe buffer" (Tris / borate / EDTA) is obtained by replacing it with boric acid. These two buffers are usually used in nucleic acid electrophoresis experiments.
TAE and tbe made from Tris are commonly used in DNA electrophoresis. Te (ph8.0) is mainly used to dissolve DNA. (TE is Tris plus EDTA.) 1mtris-hcl6.8 and 1.5mtris-hcl8.8 are commonly used in SDS-PAGE. Tris buffer has been used more and more in biochemical research, with the trend of more than phosphate buffer. Tris buffer has been used in SDS- polyacrylamide gel electrophoresis, and phosphate is rarely used. The common effective pH range of Tris buffer is in the "neutral" range.
In Tris medium, a certain microorganism can produce certain metabolites after growing in the medium, which can react with special chemicals in the medium and produce obvious characteristic changes. According to this characteristic change, this kind of microorganism can be distinguished from other microorganisms
In Tris HC, an amino group in it is a coordination group, which may replace the ligand in the original complex, thus changing the complex and affecting the absorbance. If you want to know whether there will be such an effect, you need to check their coordination constants and compare them.
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