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Wuhan Desheng Biochemical Technology Co., Ltd
Wuhan Desheng Biochemical Technology Co., Ltd
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Why does blood collection with coagulants cause hemolysis

Why does blood collection with coagulants cause hemolysis

The collection and processing of blood samples are crucial in clinical laboratory examinations. Among them, procoagulant blood collection is often used for coagulation tests and early diagnostic experiments for some diseases. However, hemolysis may occur when using blood collection vessels containing coagulants. Hemolysis is a complex process that can affect the accuracy and reliability of experimental results. This article will explore the reasons for hemolysis in blood collection using coagulants.


The components and effects of coagulants in blood collection


Coagulant agents usually contain coagulant powder (a white powder substance that needs to be prepared as a solution when used) or coagulant suspension (a milky white suspension that can be directly sprayed for use) in blood collection. The function of these two reagents is to accelerate the coagulation process of blood, causing it to quickly form clots in the blood collection vessel. This component has no effect on clinical biochemical testing and can facilitate subsequent experimental analysis.


The causes of hemolysis phenomenon


Hemolysis refers to the process in which red blood cells rupture and release intracellular substances during the preparation process after blood samples are collected from blood collection vessels. This may affect the experimental results, especially for experiments that require detection of intracellular components in red blood cells. There are various reasons for hemolysis during the use of coagulant blood collection vessels. Below, we will explore in detail some of the main reasons.


1. Improper blood collection


During the blood collection process, if the operation is improper or the blood collection technique is not proficient, it may cause damage to the blood vessel wall, leading to hemolysis. In addition, excessive compression or forceful shaking of the blood collection tube may also cause red blood cell rupture.


2. The stimulating effect of coagulants


Some coagulants may have a stimulating effect on red blood cells, leading to their rupture. When choosing products, it is important to purchase from reputable manufacturers or channels. Ensure reliable product quality.


3. Transportation and storage conditions


Improper transportation and storage conditions may cause shaking or temperature changes in the blood collection vessel, leading to hemolysis. For example, long-distance transportation or extreme temperature environments may cause the components inside the blood collection vessel to separate or produce bubbles, leading to the rupture of red blood cells.


4. Improper specimen handling


During specimen processing, if the mixing time is too long and the force is too strong, it may damage red blood cells and cause hemolysis. In addition, if the blood collection tube is not evenly filled and applied before use, it may also lead to some blood samples not fully contacting the coagulant, forming clots, while other parts continue to undergo hemolysis.


Strategies and methods to reduce hemolysis


In response to the possible causes of hemolysis mentioned above, the following strategies and methods are provided to reduce the occurrence of hemolysis:


1. Improve blood collection techniques


Strengthen training and technical guidance for blood collection personnel to enable them to master correct blood collection techniques and methods. Meanwhile, during the blood collection process, it should be kept gentle and stable, avoiding excessive force or compression of the blood collection needle.


2. Choose the appropriate coagulant


Select appropriate coagulants for different experimental needs and ensure their quality. Hubei Xindesheng Material Technology Co., Ltd. is an old brand manufacturer of blood collection additives. Their coagulants have stable quality, good usage effects, and can quickly promote blood coagulation, making them the best choice for blood collection manufacturers. It is also worth noting that the expiration date of the coagulant should be regularly checked to avoid using expired products.


3. Optimize transportation and storage conditions


Ensure that the blood collection vessel remains stable during transportation and storage, avoiding violent shaking or temperature fluctuations. For long-distance transportation of blood collection vessels, necessary shockproof and insulation measures should be taken.


4. Standardize specimen processing procedures


When handling blood samples, standardized procedures should be followed. For example, the shaking or stirring time should not be too long, and the force should not be too strong; The blood collection tube should be thoroughly shaken before use. In addition, for specimens suspected of hemolysis, timely evaluation and treatment should be carried out.


Hubei Xindesheng Material Technology Co., Ltd. was established in 2005 and is a production-oriented enterprise specializing in the research and development, production, and sales of blood testing reagents. We can provide a complete set of blood collection additive products to meet the different usage needs of customers. Stable quality and high purity, please feel free to call us for inquiries and orders if needed.